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Title: Dissemination of Walker 256 carcinoma cells to rat skeletal muscle

Abstract

After injection of 10/sup 6/ Walker 256 carcinoma cells labelled with /sup 125/I-5-iodo-2'-deoxyuridine into the tail vein, peak concentration in skeletal muscle was 46 cells/g at 60 minutes, which was lower than 169202, 1665, 555, 198 and 133 cells/g, respectively, at 30 or 60 minutes in lung, liver, spleen, kidney and heart. Because skeletal muscle constitutes 37.4% of body weight, the total number of tumor cells was 2323 cells, which was much greater than in spleen, kidney and heart with 238, 271, and 85 cells, respectively, and only less than in lung and liver, at 222857 and 11700 cells, respectively. The total number in skeletal muscle became greater than in liver at 4 hours and than in lung at 24 hours. Ten minutes after injection of 7.5 x 10/sup 6/ Walker 256 carcinoma cells into the abdominal aorta of rats, a mean of 31 colony-forming cells were recovered from the gastrocnemius, while 106 cells were recovered from the lung after injection into the tail vein. These results indicate that a large number of viable tumor cells can be arrested in skeletal muscle through circulation. The rare remote metastasis of malignancies into skeletal muscle despite constantly circulating tumor cells does notmore » appear to be due to poor dissemination of tumor cells into muscle but due to unhospitable environment of skeletal muscle.« less

Authors:
; ; ;
Publication Date:
Research Org.:
State Univ. of New York, Brooklyn
OSTI Identifier:
6990411
Report Number(s):
CONF-8604222-
Journal ID: CODEN: FEPRA
Resource Type:
Conference
Journal Name:
Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
Additional Journal Information:
Journal Volume: 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; TUMOR CELLS; BIOLOGICAL LOCALIZATION; TISSUE DISTRIBUTION; CARCINOMAS; DEOXYURIDINE; HEART; IODINE 125; KIDNEYS; LIVER; LUNGS; METASTASES; MUSCLES; RATS; SPLEEN; TRACER TECHNIQUES; ANIMAL CELLS; ANIMALS; ANTIMETABOLITES; AZINES; BETA DECAY RADIOISOTOPES; BODY; CARDIOVASCULAR SYSTEM; DAYS LIVING RADIOISOTOPES; DIGESTIVE SYSTEM; DISEASES; DISTRIBUTION; DRUGS; ELECTRON CAPTURE RADIOISOTOPES; GLANDS; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; MAMMALS; NEOPLASMS; NUCLEI; NUCLEOSIDES; NUCLEOTIDES; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; PYRIMIDINES; RADIOISOTOPES; RESPIRATORY SYSTEM; RIBOSIDES; RODENTS; URACILS; VERTEBRATES; 550901* - Pathology- Tracer Techniques

Citation Formats

Ueoka, H, Hayashi, K, Namba, T, and Grob, D. Dissemination of Walker 256 carcinoma cells to rat skeletal muscle. United States: N. p., 1986. Web.
Ueoka, H, Hayashi, K, Namba, T, & Grob, D. Dissemination of Walker 256 carcinoma cells to rat skeletal muscle. United States.
Ueoka, H, Hayashi, K, Namba, T, and Grob, D. 1986. "Dissemination of Walker 256 carcinoma cells to rat skeletal muscle". United States.
@article{osti_6990411,
title = {Dissemination of Walker 256 carcinoma cells to rat skeletal muscle},
author = {Ueoka, H and Hayashi, K and Namba, T and Grob, D},
abstractNote = {After injection of 10/sup 6/ Walker 256 carcinoma cells labelled with /sup 125/I-5-iodo-2'-deoxyuridine into the tail vein, peak concentration in skeletal muscle was 46 cells/g at 60 minutes, which was lower than 169202, 1665, 555, 198 and 133 cells/g, respectively, at 30 or 60 minutes in lung, liver, spleen, kidney and heart. Because skeletal muscle constitutes 37.4% of body weight, the total number of tumor cells was 2323 cells, which was much greater than in spleen, kidney and heart with 238, 271, and 85 cells, respectively, and only less than in lung and liver, at 222857 and 11700 cells, respectively. The total number in skeletal muscle became greater than in liver at 4 hours and than in lung at 24 hours. Ten minutes after injection of 7.5 x 10/sup 6/ Walker 256 carcinoma cells into the abdominal aorta of rats, a mean of 31 colony-forming cells were recovered from the gastrocnemius, while 106 cells were recovered from the lung after injection into the tail vein. These results indicate that a large number of viable tumor cells can be arrested in skeletal muscle through circulation. The rare remote metastasis of malignancies into skeletal muscle despite constantly circulating tumor cells does not appear to be due to poor dissemination of tumor cells into muscle but due to unhospitable environment of skeletal muscle.},
doi = {},
url = {https://www.osti.gov/biblio/6990411}, journal = {Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)},
number = ,
volume = 45:4,
place = {United States},
year = {Wed Mar 05 00:00:00 EST 1986},
month = {Wed Mar 05 00:00:00 EST 1986}
}

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