Role of TDTH and Tc populations in organ graft rejection. I. Functional analysis of graft-infiltrating T cells

Journal Article · Wed Oct 01 00:00:00 EDT 1986 · Transplantation; (United States)

OSTI ID:6989667

Stepkowski, S M; Duncan, W R

To analyze the role of T cell subpopulations in the rejection of organ allografts, we developed a new model for obtaining large numbers of graft infiltrating cells (GICs). We isolated W3/25+ Th/DTH and OX8+ Ts/c from vascularized, irradiated rat spleen allografts. W3/25+ GICs obtained from spleen allografts transplanted to normal recipients were highly effective in eliciting cardiac allograft rejection when transferred to sublethally irradiated recipients, however, the OX8+ subset was incapable of eliciting rejection. On the other hand, when OX8+ GICs were obtained from spleen allografts transplanted to previously immunized recipients, they were as efficient as the W3/25+ Th/DTH subset in eliciting cardiac allograft destruction. These results indicate that the W3/25+, OX8- T cell is required for the rejection of primary organ allografts, but that the rejection of a secondary allograft by an immune recipient may be mediated, independently, by both W3/25+ and OX8+ cells.

• Research Organization: Dalhousie Univ., Halifax, Nova Scotia
• OSTI ID: 6989667
• Journal Information: Transplantation; (United States), Vol. 4
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
GRAFT-HOST REACTION
PATHOGENESIS
BIOLOGICAL MODELS
HEART
IMMUNOSUPPRESSION
LYMPHOCYTES
MYOCARDIUM
RATS
SPLEEN
SUBLETHAL IRRADIATION
TRANSPLANTS
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARDIOVASCULAR SYSTEM
CONNECTIVE TISSUE CELLS
IRRADIATION
LEUKOCYTES
MAMMALS
MATERIALS
MUSCLES
ORGANS
RODENTS
SOMATIC CELLS
VERTEBRATES
560152* - Radiation Effects on Animals- Animals