Glycoprotein secretion in a tracheal organ culture system
Glycoprotein secretion in the rat trachea was studied in vitro, utilizing a modified, matrix embed/perfusion chamber. Baseline parameters of the culture environment were determined by enzymatic and biochemical procedures. The effect of pilocarpine on the release of labelled glycoproteins from the tracheal epithelium was assessed. After a single stimulation with the drug, there was a significant increase in the release of /sup 14/C-glucosamine and /sup 3/H-fucose-labelled glycoprotein. The response was dose-dependent. Similar results were obtained after a second exposure to pilocarpine. However, no dose response was observed. Morphological analyses of the tracheal epithelial secretory cells by Alcian Blue/Periodic Acid Schiff staining showed a significant decrease in the total number of Alcian Blue staining cells and an increase in the mixed cell population after a single exposure to pilocarpine. Second stimulation with the drug showed that the trachea was able to respond again, this time with a further decrease in the number of Alcian Blue staining cells and a decrease in the PAS staining cells as well. Carbohydrate analyses after the first simulation with pilocarpine showed increased levels of N-acetyl neuraminic acid and the neutral carbohydrates, fucose and galactose, in the precipitated glycoproteins.
- Research Organization:
- Fordham Univ., New York (USA)
- OSTI ID:
- 6985748
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
Similar Records
Histochemical and structural analysis of mucous glycoprotein secreted by the gill of Mytilus edulis
Carbohydrates of influenza virus. I. Glycopeptides derived from viral glycoproteins after labeling with radioactive sugars. [/sup 14/C, /sup 3/H]
Related Subjects
EPITHELIUM
MORPHOLOGICAL CHANGES
GLUCOPROTEINS
SECRETION
PILOCARPINE
BIOLOGICAL EFFECTS
CARBON 14 COMPOUNDS
DOSE-RESPONSE RELATIONSHIPS
GLUCOSAMINE
RATS
TISSUE CULTURES
TRACHEA
TRITIUM COMPOUNDS
ALKALOIDS
AMINES
ANIMAL TISSUES
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BODY
CARBOHYDRATES
DRUGS
HEXOSAMINES
HEXOSES
LABELLED COMPOUNDS
MAMMALS
MONOSACCHARIDES
ORGANIC COMPOUNDS
PARASYMPATHOMIMETICS
PROTEINS
RESPIRATORY SYSTEM
RODENTS
SACCHARIDES
TISSUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques
550301 - Cytology- Tracer Techniques