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Title: Distinct accessory cell requirements define two types of rat T cell hybridomas specific for unique determinants in the encephalitogenic 68-86 region of myelin basic protein

Abstract

Six clonotypically unique T cell hybridomas from Lewis rats were used to study accessory cell activities required for class II MHC restricted T cell responses to the 68-86 encephalitogenic sequence of myelin basic protein (MBP). T cell hybrids which were cultured with GP68-86 68-86 sequence of guinea pig MBP (GPMBP) and naive splenocytes (SPL) were induced to produce IL-2 as measured by the CTLL indicator cell line. The hybrids were categorized into two subsets (designated THYB-1 and THYB-2), because two distinct subset-specific pathways of communication between accessory cells and T cells were involved in GPMBP-induced IL-2 production. These pathways were distinguished by the following six observations. First, when the duration of a pulse of SPL with GPMBP was lengthened from 1 to 4 h, these SPL lost their ability to induce IL-2 production by THYB-2 hybrids yet nevertheless retained full stimulatory activity for THYB-1 hybrids. Second, paraformaldehyde fixation of GPMBP-pulsed SPL abrogated an activity necessary for Ag-induced IL-2 production by THYB-2 hybrids. These fixed SPL were nevertheless able to stimulate THYB-1 hybrids, albeit to a lesser extent than viable unfixed SPL. Third, the addition of either cycloheximide, cytochalasin B, or 2-deoxyglucose to an Ag pulse of SPL with GPMBP dramaticallymore » inhibited the subsequent responses of THYB-2 hybrids yet had little or no effect upon the reactivity of THYB-1 hybrids. Fourth, thymocytes lacked necessary activities for GPMBP evoked IL-2 production by THYB-2 hybrids yet strongly promoted THYB-1 hybrid responses. Fifth, exposure of SPL to as little as 500 rad of gamma-irradiation markedly attenuated THYB-2 hybrid response to GPMBP but did not affect THYB-1 responses. Sixth, anti-GPMBP responses by THYB-2 hybrids were observed only in the presence of both radioresistant adherent SPL and a distinct population of radiosensitive nonadherent SPL.« less

Authors:
; ; ;  [1]
  1. Univ. of Michigan Medical School, Ann Arbor (USA)
Publication Date:
OSTI Identifier:
6983462
Resource Type:
Journal Article
Journal Name:
Journal of Immunology; (USA)
Additional Journal Information:
Journal Volume: 144:2; Journal ID: ISSN 0022-1767
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; HYBRIDOMAS; SPECIFICITY; LYMPHOKINES; BIOSYNTHESIS; SPLEEN CELLS; BIOLOGICAL RADIATION EFFECTS; ANTIGENS; BIOLOGICAL PATHWAYS; CLONE CELLS; ENCEPHALITIS; GAMMA RADIATION; LYMPHOCYTES; PEPTIDES; RATS; ANIMAL CELLS; ANIMALS; BIOLOGICAL EFFECTS; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CELL CULTURES; CONNECTIVE TISSUE CELLS; DISEASES; ELECTROMAGNETIC RADIATION; GROWTH FACTORS; INFECTIOUS DISEASES; IONIZING RADIATIONS; LEUKOCYTES; MAMMALS; MATERIALS; MITOGENS; NERVOUS SYSTEM DISEASES; ORGANIC COMPOUNDS; PROTEINS; RADIATION EFFECTS; RADIATIONS; RODENTS; SOMATIC CELLS; SYNTHESIS; VERTEBRATES; VIRAL DISEASES; 560120* - Radiation Effects on Biochemicals, Cells, & Tissue Culture

Citation Formats

Mannie, M D, Paterson, P Y, Thomas, D W, and Nairn, R. Distinct accessory cell requirements define two types of rat T cell hybridomas specific for unique determinants in the encephalitogenic 68-86 region of myelin basic protein. United States: N. p., 1990. Web.
Mannie, M D, Paterson, P Y, Thomas, D W, & Nairn, R. Distinct accessory cell requirements define two types of rat T cell hybridomas specific for unique determinants in the encephalitogenic 68-86 region of myelin basic protein. United States.
Mannie, M D, Paterson, P Y, Thomas, D W, and Nairn, R. 1990. "Distinct accessory cell requirements define two types of rat T cell hybridomas specific for unique determinants in the encephalitogenic 68-86 region of myelin basic protein". United States.
@article{osti_6983462,
title = {Distinct accessory cell requirements define two types of rat T cell hybridomas specific for unique determinants in the encephalitogenic 68-86 region of myelin basic protein},
author = {Mannie, M D and Paterson, P Y and Thomas, D W and Nairn, R},
abstractNote = {Six clonotypically unique T cell hybridomas from Lewis rats were used to study accessory cell activities required for class II MHC restricted T cell responses to the 68-86 encephalitogenic sequence of myelin basic protein (MBP). T cell hybrids which were cultured with GP68-86 68-86 sequence of guinea pig MBP (GPMBP) and naive splenocytes (SPL) were induced to produce IL-2 as measured by the CTLL indicator cell line. The hybrids were categorized into two subsets (designated THYB-1 and THYB-2), because two distinct subset-specific pathways of communication between accessory cells and T cells were involved in GPMBP-induced IL-2 production. These pathways were distinguished by the following six observations. First, when the duration of a pulse of SPL with GPMBP was lengthened from 1 to 4 h, these SPL lost their ability to induce IL-2 production by THYB-2 hybrids yet nevertheless retained full stimulatory activity for THYB-1 hybrids. Second, paraformaldehyde fixation of GPMBP-pulsed SPL abrogated an activity necessary for Ag-induced IL-2 production by THYB-2 hybrids. These fixed SPL were nevertheless able to stimulate THYB-1 hybrids, albeit to a lesser extent than viable unfixed SPL. Third, the addition of either cycloheximide, cytochalasin B, or 2-deoxyglucose to an Ag pulse of SPL with GPMBP dramatically inhibited the subsequent responses of THYB-2 hybrids yet had little or no effect upon the reactivity of THYB-1 hybrids. Fourth, thymocytes lacked necessary activities for GPMBP evoked IL-2 production by THYB-2 hybrids yet strongly promoted THYB-1 hybrid responses. Fifth, exposure of SPL to as little as 500 rad of gamma-irradiation markedly attenuated THYB-2 hybrid response to GPMBP but did not affect THYB-1 responses. Sixth, anti-GPMBP responses by THYB-2 hybrids were observed only in the presence of both radioresistant adherent SPL and a distinct population of radiosensitive nonadherent SPL.},
doi = {},
url = {https://www.osti.gov/biblio/6983462}, journal = {Journal of Immunology; (USA)},
issn = {0022-1767},
number = ,
volume = 144:2,
place = {United States},
year = {Mon Jan 15 00:00:00 EST 1990},
month = {Mon Jan 15 00:00:00 EST 1990}
}