Bioactivation of diethylstilbestrol by the Syrian hamster kidney
Male Syrian golden hamsters chronically exposed to diethylstilbestrol (DES) develop renal adenocarcinomas with an incidence approaching 100%. The ability of the hamster kidney to bioactivate DES was assessed using hamster kidney slices. The male hamster renal cortex has a 2- to 5-fold greater capacity to irreversibly bind ({sup 3}H)DES as compared with female hamster renal cortex and with male hamster renal medulla. Incubation of the tissue under anaerobic conditions inhibited the metabolism and irreversible binding of ({sup 3}H)DES. Gel electrophoresis analysis of covalently modified proteins revealed several radioactive peaks indicating that specific adduct formation had occurred. The cytochrome P-450 inhibitors SKF 525-A, metyrapone, carbon monoxide, butylated hydroxytoluene, and dicumarol decreased the irreversible binding of ({sup 3}H)DES to renal cortical protein by 38 to 72%.
- Research Organization:
- Rochester Univ., NY (USA)
- OSTI ID:
- 6982889
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
Similar Records
In vitro adrenal bioactivation and effects on steroid metabolism of DDT, PCBs and their metabolites in the gray seal (Halichoerus grypus)
An epoxysuccinic acid derivative(loxistatin)-induced hepatic injury in rats and hamsters
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
STILBESTROL
METABOLISM
BIOCHEMICAL REACTION KINETICS
CHRONIC EXPOSURE
DNA ADDUCTS
ELECTROPHORESIS
ENZYME INHIBITORS
HAMSTERS
KIDNEYS
SEX DEPENDENCE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ADDUCTS
ANIMALS
AROMATICS
BODY
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
MAMMALS
ORGANIC COMPOUNDS
ORGANS
PHENOLS
POLYPHENOLS
REACTION KINETICS
RODENTS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology
550501 - Metabolism- Tracer Techniques