Assignment of the human tear lipocalin gene (LCN1) to 9q34 by in situ hybridization
- Laboratoire de Biochimie Medicale, Clermont-Ferrand (France)
- Groupe Hospitalier de la Timone, Marseille (France)
Lipocalins are a group of extracellular proteins, first described by Pervaiz and Brew, that are able to bind lipophiles by enclosure within their structures, minimizing solvent contact. A cDNA library was constructed from human lacrimal glands obtained post-mortem, after extraction of total RNA by phenol chloroform and purification of poly(A)[sup +] RNA by an oligo(dT) column. The library was screened using an oligonucleotide probe whose sequence was deduced from the already mentioned N-terminal sequence. Sequencing one of the positive clones (clone 16) allowed us to determine the main characteristics of the protein and to confirm that it belongs to the lipocalin family. This results was assessed, at the same time, by another independent team that demonstrated that this protein is also present in saliva. We report the use of clone 16 cDNA to map the tear lipocalin gene by in situ hybridization. The results from the best experiment are as follows: In the 100 metaphase cells examined after in situ hybridization, there were 148 silver grains associated with chromosomes, and 56 (37.8%) were located on chromosome 9. The distribution of the grains on this chromosome was not random: 46/56 (82.1%) of them mapped to the q34.1-q34.3 region of chromosome 9 with a maximum in the q34.3 band. No other chromosomal region was observed to have signal above background. These results allow us to map the tear lipocalin probe to human chromosome 9q34.
- OSTI ID:
- 6975396
- Journal Information:
- Genomics; (United States), Vol. 18:1; ISSN 0888-7543
- Country of Publication:
- United States
- Language:
- English
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