Solubilization and functional reconstitution of polymorphonuclear leukocyte formyl-Methionyl-Leucyl-Phenylalanine receptors and guanine nucleotide binding proteins
Formyl-Methionyl-Leucyl-Phenylalanine (fMLP) binds to specific polymorphonuclear leukocyte plasma membrane receptors stimulating chemotaxis and bactericidal responses. One of the initial events of the ligand receptor interaction is a rise in inositol trisphosphate, which triggers intracellular calcium release. The generation of inositol trisphosphate is mediated by the fMLP-activated phospholipase C via a GTP-binding protein (G-protein). In analogy to the adrenergic stimulation of adenylate cyclase, the following signal transduction model has been proposed: The fMLP receptor activates a G-protein which then stimulates phospholipase C to hydrolyse phosphatidylinositol bisphosphate to inositol trisphosphate and diacylglycerol. This work has focused on characterizing the structural and functional coupling fMLP receptor and G-proteins in native membranes, detergent micelles and reconstituted phospholipid vesicles. Tight coupling between the fMLP receptor and G-protein has been demonstrated in both native and solubilized membranes by assaying quanine nucleotide-induced inhibition of (/sup 3/H)fMLP binding and fMLP stimulated GTPase activity.
- Research Organization:
- Boston Univ., MA (USA)
- OSTI ID:
- 6974765
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
LIPASE
ENZYME ACTIVITY
PHENYLALANINE
BIOCHEMICAL REACTION KINETICS
RECEPTORS
PHOSPHOLIPIDS
BIOSYNTHESIS
SOLUBILITY
CELL MEMBRANES
GUANINE
LEUKOCYTES
NUCLEOTIDES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
AMINES
AMINO ACIDS
AROMATICS
AZAARENES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLESTERASES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
ENZYMES
ESTERASES
ESTERS
HETEROCYCLIC COMPOUNDS
HYDROLASES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LIPIDS
MATERIALS
MEMBRANE PROTEINS
MEMBRANES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PROTEINS
PURINES
REACTION KINETICS
SYNTHESIS
550201* - Biochemistry- Tracer Techniques