Urinary 8-hydroxy-2 prime -deoxyguanosine as a biological marker of in vivo oxidative DNA damage

Journal Article · Fri Dec 01 00:00:00 EST 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.24.9697· OSTI ID:6970174

Shigenaga, M K; Gimeno, C J; Ames, B N ^[1]

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1. Univ. of California, Berkeley (USA)

DNA is subject to constant oxidative damage from endogenous oxidants. The oxidized DNA is continuously repaired and the oxidized bases are excreted in the urine. A simple routine analytical procedure is described for urinary 8-hydroxy-2{prime}-deoxyguanosine, an oxidative DNA damage adduct, as an indicator of oxidative damage in humans and rodents. This adduct was purified from human urine and characterized. The described assay employs a series of solid-phase extraction steps that separate 8-hydroxy-2{prime}-deoxyguanosine from other urinary constituents, followed by analysis by gradient reversed-phase HPLC coupled to a dual-electrode high-efficient electrochemical detection system. Analysis of urine from three species by this method indicates that mice excrete approximately 3.3-fold more 8-hydroxy-2{prime}-deoxyguanosine than humans (582 vs. 178 residues per cell day), a result that supports the proposal that oxidative damage to DNA increases in proportion to species-specific basal metabolic rates.

• OSTI ID: 6970174
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 86:24; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
DNA
OXIDATION
DNA ADDUCTS
EXCRETION
DNA REPAIR
BIOLOGICAL MARKERS
GUANOSINE
LIQUID COLUMN CHROMATOGRAPHY
METABOLISM
PURIFICATION
TRITIUM COMPOUNDS
ULTRAVIOLET SPECTRA
URINE
ADDUCTS
AROMATICS
AZAARENES
BIOLOGICAL MATERIALS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOLOGICAL WASTES
BODY FLUIDS
CHEMICAL REACTIONS
CHROMATOGRAPHY
CLEARANCE
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
MATERIALS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PURINES
RECOVERY
REPAIR
RIBOSIDES
SEPARATION PROCESSES
SPECTRA
WASTES
550201* - Biochemistry- Tracer Techniques