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Title: In vivo sup 23 Na and sup 31 P NMR measurement of a tonoplast Na sup + /H sup + exchange process and its characteristics in two barley cultivars

Abstract

A Na{sup +} uptake-associated vacuolar alkalinization was observed in roots of two barley cultivars (Arivat and the more salt-tolerant California Mariout) by using {sup 23}Na and {sup 31}P in vivo NMR spectroscopy. A NaCl uptake-associated broadening was also noted for both vacuolar P{sub i} and intracellular Na NMR peaks, consistent with Na{sup +} uptake into the same compartment as the vacuolar P{sub i}. A close coupling of Na{sup +} with H{sup +} transport (presumably the Na{sup +}/H{sup +} antiport) in vivo was evidence by qualitative and quantitative correlations between Na{sup +} accumulation and vacuolar alkalinization for both cultivars. Prolongation of the low NaCl pretreatment (30 mM) increased the activity of the putative antiport in Arivat but reduced it in California Mariout. This putative antiport also showed a dependence on NaCl concentration for California Mariout but not for Arivat. No cytoplasmic acidification accompanied the antiporter activity for either cultivar. The response of adenosine phosphates indicated that ATP utilization exceeded the capacity for ATP synthesis in Arivat, but the two processes seemed balanced in California Mariout. These comparisons provide clues to the role of the tonoplast Na{sup +}/H{sup +} antiport and compensatory cytoplasmic adjustments including pH, osymolytes, and energy phosphates in governingmore » the different salt tolerance of the two cultivars.« less

Authors:
; ;  [1];  [2]
  1. Univ. of California, Davis (USA)
  2. Univ. of California, Bodega Bay (USA)
Publication Date:
OSTI Identifier:
6969404
Resource Type:
Journal Article
Journal Name:
Proceedings of the National Academy of Sciences of the United States of America; (USA)
Additional Journal Information:
Journal Volume: 86:24; Journal ID: ISSN 0027-8424
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; IONS; MEMBRANE TRANSPORT; MEMBRANE PROTEINS; NUCLEAR MAGNETIC RESONANCE; ATP; BARLEY; PH VALUE; PHOSPHORUS 31; PROTONS; SEEDS; SODIUM 23; SODIUM CHLORIDES; ALKALI METAL COMPOUNDS; ALKALI METAL ISOTOPES; BARYONS; CEREALS; CHARGED PARTICLES; CHLORIDES; CHLORINE COMPOUNDS; ELEMENTARY PARTICLES; FERMIONS; GRASS; HADRONS; HALIDES; HALOGEN COMPOUNDS; ISOTOPES; LIGHT NUCLEI; LILIOPSIDA; MAGNETIC RESONANCE; MAGNOLIOPHYTA; NUCLEI; NUCLEONS; NUCLEOTIDES; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; PHOSPHORUS ISOTOPES; PLANTS; PROTEINS; RESONANCE; SODIUM COMPOUNDS; SODIUM ISOTOPES; STABLE ISOTOPES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics

Citation Formats

Fan, T W.M., Norlyn, J, Epstein, E, and Higashi, R M. In vivo sup 23 Na and sup 31 P NMR measurement of a tonoplast Na sup + /H sup + exchange process and its characteristics in two barley cultivars. United States: N. p., 1989. Web. doi:10.1073/pnas.86.24.9856.
Fan, T W.M., Norlyn, J, Epstein, E, & Higashi, R M. In vivo sup 23 Na and sup 31 P NMR measurement of a tonoplast Na sup + /H sup + exchange process and its characteristics in two barley cultivars. United States. https://doi.org/10.1073/pnas.86.24.9856
Fan, T W.M., Norlyn, J, Epstein, E, and Higashi, R M. 1989. "In vivo sup 23 Na and sup 31 P NMR measurement of a tonoplast Na sup + /H sup + exchange process and its characteristics in two barley cultivars". United States. https://doi.org/10.1073/pnas.86.24.9856.
@article{osti_6969404,
title = {In vivo sup 23 Na and sup 31 P NMR measurement of a tonoplast Na sup + /H sup + exchange process and its characteristics in two barley cultivars},
author = {Fan, T W.M. and Norlyn, J and Epstein, E and Higashi, R M},
abstractNote = {A Na{sup +} uptake-associated vacuolar alkalinization was observed in roots of two barley cultivars (Arivat and the more salt-tolerant California Mariout) by using {sup 23}Na and {sup 31}P in vivo NMR spectroscopy. A NaCl uptake-associated broadening was also noted for both vacuolar P{sub i} and intracellular Na NMR peaks, consistent with Na{sup +} uptake into the same compartment as the vacuolar P{sub i}. A close coupling of Na{sup +} with H{sup +} transport (presumably the Na{sup +}/H{sup +} antiport) in vivo was evidence by qualitative and quantitative correlations between Na{sup +} accumulation and vacuolar alkalinization for both cultivars. Prolongation of the low NaCl pretreatment (30 mM) increased the activity of the putative antiport in Arivat but reduced it in California Mariout. This putative antiport also showed a dependence on NaCl concentration for California Mariout but not for Arivat. No cytoplasmic acidification accompanied the antiporter activity for either cultivar. The response of adenosine phosphates indicated that ATP utilization exceeded the capacity for ATP synthesis in Arivat, but the two processes seemed balanced in California Mariout. These comparisons provide clues to the role of the tonoplast Na{sup +}/H{sup +} antiport and compensatory cytoplasmic adjustments including pH, osymolytes, and energy phosphates in governing the different salt tolerance of the two cultivars.},
doi = {10.1073/pnas.86.24.9856},
url = {https://www.osti.gov/biblio/6969404}, journal = {Proceedings of the National Academy of Sciences of the United States of America; (USA)},
issn = {0027-8424},
number = ,
volume = 86:24,
place = {United States},
year = {Fri Dec 01 00:00:00 EST 1989},
month = {Fri Dec 01 00:00:00 EST 1989}
}