Use of indium-111 oxine to study pulmonary and hepatic leukocyte sequestration in endotoxin shock and effects of the beta-2 receptor agonist terbutaline
- Kuwait Univ., Safat (Kuwait)
The dynamic behavior of indium-111 oxine-labeled leukocytes was simultaneously recorded in multiple organs during endotoxin shock in sheep. Also, the effects of the beta-2 receptor agonist terbutaline were studied. An experimental protocol was designed to mimic a clinical condition in an intensive care setting as far as possible. The animals were ventilated with 50% oxygen to avoid hypoxemia and were given large amounts of intravenous fluids to reduce adverse effects of hypovolemia. A moderate dose of E. coli endotoxin (10 micrograms/kg bwt) was given by intravenous infusion to 14 adult sheep, seven of them receiving continuous intravenous infusion of terbutaline (20 micrograms/kg/hr) during 4 hr, starting 30 min after endotoxin, when signs of lung injury had developed. The other seven acted as controls. A marked pulmonary and hepatic leukocyte sequestration together with a sharp drop in leukocyte counts in peripheral blood occurred within minutes after start of the endotoxin infusion in both groups. However, no changes were observed in the kidneys or the gut. After 60 min and until the end of the experiment, there was a significantly lower activity in the lungs and in the liver of the animals treated with terbutaline than in the controls (P less than .01). Furthermore, less marked hemodynamic and respiratory alterations occurred in the terbutaline group compared with the controls. This study confirms the results of other investigators showing that significant leukocyte sequestration occurs in the lungs during endotoxemia, but it also demonstrates that leukocytes sequestrate in the liver, although slightly less than in the lungs.
- OSTI ID:
- 6969387
- Journal Information:
- American Journal of Physiologic Imaging; (USA), Vol. 4:4; ISSN 0885-8276
- Country of Publication:
- United States
- Language:
- English
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BACTERIAL DISEASES
DIAGNOSIS
LEUKOCYTES
TISSUE DISTRIBUTION
SYMPATHOMIMETICS
RECEPTORS
ENDOTOXINS
ESCHERICHIA COLI
IMAGE PROCESSING
INDIUM 111
LABELLING
LIVER
LUNGS
ORGANOMETALLIC COMPOUNDS
SHEEP
ANIMALS
ANTIGENS
AUTONOMIC NERVOUS SYSTEM AGENTS
BACTERIA
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISEASES
DISTRIBUTION
DOMESTIC ANIMALS
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
GLANDS
INDIUM ISOTOPES
INFECTIOUS DISEASES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MAMMALS
MATERIALS
MEMBRANE PROTEINS
MICROORGANISMS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROCESSING
PROTEINS
RADIOISOTOPES
RESPIRATORY SYSTEM
RUMINANTS
TOXIC MATERIALS
TOXINS
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics