H{sub 2} photoproduction by batch culture of Anabaena variabilis ATCC 29413 and its mutant PK84 in a photobioreactor
- Kings Coll. London (United Kingdom)
Hydrogen production by Anabaena variabilis ATCC 29413 and of its mutant PK84, grown in batch cultures, was studied in a photobioreactor. The highest volumetric H{sub 2} production rates of native and mutant strains were found in cultures grown at gradually increased irradiation. The native strain evolved H{sub 2} only under an argon atmosphere with the actual rate as high as the potential rate. In this case 61% of oxygenic photosynthesis was used for H{sub 2}production. In contrast the mutant PK84 produced H{sub 2} during growth under CO{sub 2}-enriched air. Under these conditions at the maximum rate of H{sub 2} production, 13% of oxygenic photosynthesis was used for H{sub 2} production and the actual H{sub 2} production was only 22% of the potential. Under an atmosphere of 98% argon + 2% CO{sub 2} actual H{sub 2} by the mutant was strictly light-dependent with saturation at about 300{mu}E m{sup {minus}2}s{sup {minus}1}. However, the rate of photosynthesis was not saturated at this irradiation. At limiting light intensities 33--58% of photosynthesis was used for J{sub 2} production. Hydrogen evolution by PK84 under air + 2% CO{sub 2} was also stimulated by light; but was not saturated at 332 {mu}E m{sup {minus}2} s{sup {minus}1} and did not cease completely in darkness. The rate of oxygen photoevolution was also not saturated. A mechanism for increasing cyanobacterial hydrogen production is proposed.
- OSTI ID:
- 696814
- Journal Information:
- Biotechnology and Bioengineering, Vol. 64, Issue 6; Other Information: PBD: 20 Sep 1999
- Country of Publication:
- United States
- Language:
- English
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