Sustained bacterial reduction of Co{sup III}EDTA{sup {minus}} in the presence of competing geochemical oxidation during dynamic flow

Brooks, S C; Carroll, S L; Jardine, P M

doi:10.1021/es990245f

Title: Sustained bacterial reduction of Co{sup III}EDTA{sup {minus}} in the presence of competing geochemical oxidation during dynamic flow

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Abstract

Radionuclides and metals can be mobilized by chelating agents typically present in low-level radioactive liquid wastes. [{sup 60}Co]EDTA in the form {sup 60}Co{sup III}EDTA{sup {minus}} represents a highly stable and mobile form of this radionuclide. By contract, the reduced form of this metal-ligand complex, {sup 60}Co{sup II}EDTA{sup 2{minus}}, is much less stable and less mobile. There is an increasing awareness that dissimilatory metal-reducing bacteria (DMRB) can be used to mediate redox transformations of metals and radionuclides whose stability and mobility are governed by their oxidation state. The authors conducted a series of column experiments to provide an improved understanding of Co{sup III}EDTA{sup {minus}} reduction by the facultative anaerobe Shewanella alga BrY (BrY). Experiments were conducted under growth conditions using lactate a carbon and energy source. They were able to demonstrate the sustained reduction of Co{sup III}EDTA{sup {minus}} in column flow experiments with the desired result that a less stable, less mobile product was formed. The amount of reduction varied directly with the fluid residence time in the columns. In the presence of a suitable mineral sorbent [Fe(OH){sub 3}], Co-EDTA transport was delayed as a direct consequence of the bacterial reduction reaction. Even in the presence of a strong mineral oxidantmore »« less

Authors:

Brooks, S C; Carroll, S L; Jardine, P M ^[1]

Oak Ridge National Lab., TN (United States). Environmental Sciences Div.

Publication Date:: Wed Sep 01 00:00:00 EDT 1999

Sponsoring Org.:: USDOE, Washington, DC (United States)

OSTI Identifier:: 696774

DOE Contract Number:: AC05-96OR22464

Resource Type:: Journal Article

Journal Name:: Environmental Science and Technology

Additional Journal Information:: Journal Volume: 33; Journal Issue: 17; Other Information: PBD: 1 Sep 1999

Country of Publication:: United States

Language:: English

Subject:: 54 ENVIRONMENTAL SCIENCES; COBALT 60; EDTA; BIODEGRADATION; REMEDIAL ACTION; ENVIRONMENTAL TRANSPORT; LOW-LEVEL RADIOACTIVE WASTES; REDOX REACTIONS; GEOCHEMISTRY

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                    Brooks, S C, Carroll, S L, and Jardine, P M. Sustained bacterial reduction of Co{sup III}EDTA{sup {minus}} in the presence of competing geochemical oxidation during dynamic flow.  United States: N. p., 1999. 
        Web.  doi:10.1021/es990245f.

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                    Brooks, S C, Carroll, S L, & Jardine, P M. Sustained bacterial reduction of Co{sup III}EDTA{sup {minus}} in the presence of competing geochemical oxidation during dynamic flow.  United States.  https://doi.org/10.1021/es990245f

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                    Brooks, S C, Carroll, S L, and Jardine, P M. 1999.  
        "Sustained bacterial reduction of Co{sup III}EDTA{sup {minus}} in the presence of competing geochemical oxidation during dynamic flow".  United States.  https://doi.org/10.1021/es990245f.

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@article{osti_696774,

  title        = {Sustained bacterial reduction of Co{sup III}EDTA{sup {minus}} in the presence of competing geochemical oxidation during dynamic flow},

  author       = {Brooks, S C and Carroll, S L and Jardine, P M},

  abstractNote = {Radionuclides and metals can be mobilized by chelating agents typically present in low-level radioactive liquid wastes. [{sup 60}Co]EDTA in the form {sup 60}Co{sup III}EDTA{sup {minus}} represents a highly stable and mobile form of this radionuclide. By contract, the reduced form of this metal-ligand complex, {sup 60}Co{sup II}EDTA{sup 2{minus}}, is much less stable and less mobile. There is an increasing awareness that dissimilatory metal-reducing bacteria (DMRB) can be used to mediate redox transformations of metals and radionuclides whose stability and mobility are governed by their oxidation state. The authors conducted a series of column experiments to provide an improved understanding of Co{sup III}EDTA{sup {minus}} reduction by the facultative anaerobe Shewanella alga BrY (BrY). Experiments were conducted under growth conditions using lactate a carbon and energy source. They were able to demonstrate the sustained reduction of Co{sup III}EDTA{sup {minus}} in column flow experiments with the desired result that a less stable, less mobile product was formed. The amount of reduction varied directly with the fluid residence time in the columns. In the presence of a suitable mineral sorbent [Fe(OH){sub 3}], Co-EDTA transport was delayed as a direct consequence of the bacterial reduction reaction. Even in the presence of a strong mineral oxidant the net reduction of Co{sup III}EDTA{sup {minus}} dominated the fate and transport of this species.},

  doi          = {10.1021/es990245f},

  url          = {https://www.osti.gov/biblio/696774},
  journal      = {Environmental Science and Technology},
number       = 17,

  volume       = 33,

  place        = {United States},

  year         = {Wed Sep 01 00:00:00 EDT 1999},

  month        = {Wed Sep 01 00:00:00 EDT 1999}

}

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