Organization of the human T cell receptor [zeta]/[eta] gene and its genetic linkage to the Fc[gamma]RII-Fc[gamma]RIII gene cluster

Journal Article · Wed Apr 15 00:00:00 EDT 1992 · Journal of Immunology; (United States)

OSTI ID:6965090

Jensen, J P; Hou, D; Taylor, A ^[1]; Dean, M ^[2]; Ramsburg, M ^[3]; Weissman, A W ^[4]

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1. DCBDC/National Cancer Institute National Institutes of Health, Bethesda, MD (United States)
2. National Cancer Institute, FCRDC, National Institute of Health, Frederick, MD (United States)
3. Program Resources Inc./DynCorp, FCRDC, Frederick, MD (United States)
4. DCBDC/NCI NIH, Bethesda, MD (United States) DCBDC/National Cancer Institute National Institute of Health, Bethesda, MD (United States)

The [zeta]-subunit is the most recently characterized stoichiometric human TCR component. In this study the authors describe the molecular organization of the human [zeta]-gene. The [zeta] transcript is generated as the spliced product of eight exons that are separated by distances of 0.7 kb to more than 8 kb. Ribonuclease protection studies revealed multiple transcription initiation sites distributed over a range of approximately 115 bases. A variable number tandem repeat restriction fragment polymorphism contained within the structural gene has allowed for the localization of [zeta] within the human genome. Additionally, a restriction fragment polymorphism within the Fc[gamma]RII-Fc[gamma]RIII gene cluster has allowed for its localization on the map of human chromosome 1q and for the establishment of its linkage to the [zeta]-gene locus. A region that is highly homologous on a nucleotide level with the [eta]-exon of the murine [zeta]-gene is localized to the 3[prime] region of the human [zeta]-gene. Surprisingly, translation of this region into protein results in a structure that is markedly divergent from its murine counterpart. This finding has important implications regarding the potential role of [eta] in T cell function. 60 refs., 7 figs., 1 tab.

• OSTI ID: 6965090
• Journal Information: Journal of Immunology; (United States), Vol. 148:8; ISSN 0022-1767
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
HUMAN CHROMOSOME 1
GENETIC MAPPING
LYMPHOCYTES
RECEPTORS
GENES
ANIMAL CELLS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHROMOSOMES
CONNECTIVE TISSUE CELLS
HUMAN CHROMOSOMES
LEUKOCYTES
MAPPING
MATERIALS
MEMBRANE PROTEINS
ORGANIC COMPOUNDS
PROTEINS
SOMATIC CELLS
550400* - Genetics