Interactions of radiation and 5-fluorouracil, cyclophosphamide or methotrexate in intestinal crypt cells
The interactions of radiation and 5-fluorouracil (5-FU), cyclophosphamide (CTX), or methotrexate (MTX) in mouse jejunal crypt cells were studied using the microcolony survival assay. 5-FU given from 48 hr before to 24 hr after irradiation resulted in an almost constant, increased cell kill except at injection 6 hr after irradiation, which resulted in a more pronounced effect. CTX enhanced the radiation effect only when given simultaneously with or up to 3 hr after irradiation. The effect of MTX, extremely dependent on the sequence and interval between drug administration and irradiation, was most prominent when administered 1 hr before irradiation. At this drug-radiation interval, the D/sub 0/ surprisingly increased by a factor of 2.4, whereas MTX 15 min before irradiation displaced the survival curve to the left without changing the D/sub 0/. The influence of MTX on the radiation response disappeared when the drug was given either 96 hr before or 3 hr after irradiation.
- Research Organization:
- Inst. of Cancer Research, Aarhus, Denmark
- OSTI ID:
- 6964094
- Journal Information:
- Int. J. Radiat. Oncol., Biol. Phys.; (United States), Vol. 12:1
- Country of Publication:
- United States
- Language:
- English
Similar Records
Response of murine jejunal crypts to single doses of cyclophosphamide and radiation. [Mice; whole-body. gamma. irradiation]
Studies of variation in inherent sensitivities to radiation, 5-fluorouracil and methotrexate in a series of human and murine tumor cell lines in vitro
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
CELL KILLING
RADIOINDUCTION
ENDOXAN
RADIOSENSITIVITY EFFECTS
FLUOROURACILS
METHOTREXATE
CRYPT CELLS
MICE
SURVIVAL CURVES
X RADIATION
ALKYLATING AGENTS
ANIMAL CELLS
ANIMALS
ANTIMETABOLITES
AZINES
DRUGS
ELECTROMAGNETIC RADIATION
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IMMUNOSUPPRESSIVE DRUGS
IONIZING RADIATIONS
MAMMALS
ORGANIC COMPOUNDS
ORGANIC FLUORINE COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RADIATIONS
RODENTS
SOMATIC CELLS
URACILS
VERTEBRATES
560121* - Radiation Effects on Cells- External Source- (-1987)
550300 - Cytology