Analysis of the Cu, Fe, and Zn contents in cytochrome C oxidases from different species and tissues by proton-induced X-ray emission (PIXE)

Journal Article · Fri Nov 14 00:00:00 EST 1986 · Biochem. Biophys. Res. Commun.; (United States)

DOI:https://doi.org/10.1016/0006-291X(86)90735-7· OSTI ID:6962551

Bombelka, E; Richter, F W; Stroh, A; Kadenbach, B

The Cu, Fe and Zn contents in isolated cytochrome c oxidase preparations from heart, liver, diaphragm or kidney of bovine, pig and rat was measured by proton-induced X-ray emission (PIXE). The average Cu/2Fe ratio was 2.73 and Zn/2Fe ratio 0.98. Correspondingly a Cu/Zn ratio of 2.76 was found. Dialysis of the bovine heart enzyme against increasing EDTA concentrations up to 30 mM did not change this result. It is concluded that all isozymes of mammalian cytochrome c oxidase contain 3 Cu, 2 Fe and 1 Zn per monomeric catalytic unit.

• Research Organization: Philipps Universitaet, Marburg, Germany, F.R.
• OSTI ID: 6962551
• Journal Information: Biochem. Biophys. Res. Commun.; (United States), Vol. 3
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
COPPER
PIXE ANALYSIS
CYTOCHROME OXIDASE
CHEMICAL ANALYSIS
IRON
ZINC
CATTLE
LIVER
MUSCLES
MYOCARDIUM
PROTONS
RATS
SWINE
ANIMALS
BARYONS
BODY
CARDIOVASCULAR SYSTEM
DIGESTIVE SYSTEM
DOMESTIC ANIMALS
ELEMENTARY PARTICLES
ELEMENTS
ENZYMES
FERMIONS
GLANDS
HADRONS
HAEM DEHYDROGENASES
HEART
MAMMALS
METALS
NONDESTRUCTIVE ANALYSIS
NUCLEONS
ORGANS
OXIDOREDUCTASES
RODENTS
RUMINANTS
TRANSITION ELEMENTS
VERTEBRATES
X-RAY EMISSION ANALYSIS
560300* - Chemicals Metabolism & Toxicology