Affinity purifications of aldose reductase and xylitol dehydrogenase from the xylose-fermenting yeast Pachysolen tannophilus
Journal Article
·
· Appl. Environ. Microbiol.; (United States)
OSTI ID:6959280
Although xylose is a major product of hydrolysis of lignocellulosic materials, few yeasts are able to convert it to ethanol. In Pachysolen tannophilus, one of the few xylose-fermenting yeasts found, aldose reductase and xylitol dehydrogenase were found to be key enzymes in the metabolic pathway for xylose fermentation. This paper presents a method for the rapid and simultaneous purification of both aldose reductase and xylitol dehydrogenase from P. tannophilus. Preliminary studies indicate that this method may be easily adapted to purify similar enzymes from other xylose-fermenting yeasts.
- Research Organization:
- Dept. of Agriculture, Peoria, IL
- OSTI ID:
- 6959280
- Journal Information:
- Appl. Environ. Microbiol.; (United States), Vol. 52:4
- Country of Publication:
- United States
- Language:
- English
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·
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Related Subjects
09 BIOMASS FUELS
59 BASIC BIOLOGICAL SCIENCES
OXIDOREDUCTASES
PURIFICATION
XYLOSE
FERMENTATION
METABOLISM
YEASTS
BIOLOGICAL PATHWAYS
ALDEHYDES
BIOCONVERSION
CARBOHYDRATES
ENZYMES
FUNGI
MICROORGANISMS
MONOSACCHARIDES
ORGANIC COMPOUNDS
PENTOSES
PLANTS
SACCHARIDES
090222* - Alcohol Fuels- Preparation from Wastes or Biomass- (1976-1989)
550500 - Metabolism
59 BASIC BIOLOGICAL SCIENCES
OXIDOREDUCTASES
PURIFICATION
XYLOSE
FERMENTATION
METABOLISM
YEASTS
BIOLOGICAL PATHWAYS
ALDEHYDES
BIOCONVERSION
CARBOHYDRATES
ENZYMES
FUNGI
MICROORGANISMS
MONOSACCHARIDES
ORGANIC COMPOUNDS
PENTOSES
PLANTS
SACCHARIDES
090222* - Alcohol Fuels- Preparation from Wastes or Biomass- (1976-1989)
550500 - Metabolism