Electrophoretic detection of protein p53 in human leukocytes

Journal Article · Wed Jan 01 00:00:00 EST 1986 · Bull. Exp. Biol. Med. (Engl. Transl.); (United States)

OSTI ID:6958298

Paponov, V D; Kupsik, E G; Shcheglova, E G; Yarullin, N N

The authors have found an acid-soluble protein with mol. wt. of about 53 kD in peripheral blood leukocytes of persons with Down's syndrome. It was present in different quantities in all 20 patients tested, but was virtually not discovered in 12 healthy blood donors. This paper determines the possible identity of this protein with protein p53 from mouse ascites carcinoma by comparing their electrophoretic mobilities, because the accuracy of electrophoretic determination of the molecular weight of proteins is not sufficient to identify them. The paper also describes experiments to detect a protein with electrophoretic mobility identical with that of a protein in the leukocytes of patients with Down's syndrome in leukocytes of patients with leukemia. To discover if protein p53 is involved in cell proliferation, the protein composition of leukocytes from healthy blood donors, cultured in the presence and absence of phytohemagglutinin (PHA), was compared. Increased incorporation of H 3-thymidine by leukocytes of patients with Down's syndrome is explained by the presence of a population of immature leukocytes actively synthesizing DNA in the peripheral blood of these patients, and this can also explain the presence of protein p53 in the leukocytes of these patients.

• Research Organization: Institute of Medical Genetics, Academy of Medical Sciences of the USSR, Moscow
• OSTI ID: 6958298
• Journal Information: Bull. Exp. Biol. Med. (Engl. Transl.); (United States), Vol. 100:8
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ASCITES TUMOR CELLS
TRACER TECHNIQUES
LEUKOCYTES
PATIENTS
DOWNS SYNDROME
LEUKEMIA
PROTEINS
ELECTROPHORESIS
UPTAKE
BIOSYNTHESIS
CELL PROLIFERATION
COMPARATIVE EVALUATIONS
DNA
PHYTOHEMAGGLUTININ
RETENTION
THYMIDINE
TRITIUM COMPOUNDS
AGGLUTININS
ANIMAL CELLS
ANTIBODIES
AZINES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CONGENITAL MALFORMATIONS
DISEASES
HEMAGGLUTININS
HEMIC DISEASES
HEREDITARY DISEASES
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MALFORMATIONS
MATERIALS
MITOGENS
MUCOPROTEINS
NEOPLASMS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PATHOLOGICAL CHANGES
POLYSACCHARIDES
PYRIMIDINES
RIBOSIDES
SACCHARIDES
SYNTHESIS
TUMOR CELLS
550301* - Cytology- Tracer Techniques
550501 - Metabolism- Tracer Techniques