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Title: Determination of the average orientation of DNA in the octopus sperm [ital Eledone] [ital cirrhossa] through polarized light scattering

Abstract

The coupled-dipole approximation has been used to model polarized light-scattering data obtained from the sperm of the octopus [ital Eledone] [ital cirrhosa]. Mueller scattering-matrix elements (which describe how a sample alters the intensity and degree of polarization of scattered light) were measured as a function of angle. The sample was modeled as a helical fiber believed to correspond to a DNA protein complex. It was necessary to propose an inherent anisotropy in the polarizability of the fiber in order to fit the data. The direction of the principle axes of the polarizability were determined by comparing the model with experimental data. The results suggest that the 2-nm DNA fibers are perpendicular to the thick fiber that defines the helical geometry of the octopus sperm head.

Authors:
; ; ; ; ; ; ;  [1]
  1. University of California, Berkeley, Berkeley, California 94720 (United States)
Publication Date:
OSTI Identifier:
6958288
DOE Contract Number:  
AC03-76SF00098
Resource Type:
Journal Article
Journal Name:
Applied Optics; (United States)
Additional Journal Information:
Journal Volume: 33:24; Journal ID: ISSN 0003-6935
Country of Publication:
United States
Language:
English
Subject:
42 ENGINEERING; DNA; POLARIZABILITY; SPERMATOZOA; LIGHT SCATTERING; ANISOTROPY; CHROMATIN; POLARIZATION; ELECTRICAL PROPERTIES; GAMETES; GERM CELLS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; PHYSICAL PROPERTIES; SCATTERING; 426002* - Engineering- Lasers & Masers- (1990-)

Citation Formats

Shapiro, D B, Maestre, M F, McClain, W M, Hull, P G, Shi, Y, Quinby-Hunt, M S, Hearst, J E, and Hunt, A J. Determination of the average orientation of DNA in the octopus sperm [ital Eledone] [ital cirrhossa] through polarized light scattering. United States: N. p., 1994. Web. doi:10.1364/AO.33.005733.
Shapiro, D B, Maestre, M F, McClain, W M, Hull, P G, Shi, Y, Quinby-Hunt, M S, Hearst, J E, & Hunt, A J. Determination of the average orientation of DNA in the octopus sperm [ital Eledone] [ital cirrhossa] through polarized light scattering. United States. https://doi.org/10.1364/AO.33.005733
Shapiro, D B, Maestre, M F, McClain, W M, Hull, P G, Shi, Y, Quinby-Hunt, M S, Hearst, J E, and Hunt, A J. 1994. "Determination of the average orientation of DNA in the octopus sperm [ital Eledone] [ital cirrhossa] through polarized light scattering". United States. https://doi.org/10.1364/AO.33.005733.
@article{osti_6958288,
title = {Determination of the average orientation of DNA in the octopus sperm [ital Eledone] [ital cirrhossa] through polarized light scattering},
author = {Shapiro, D B and Maestre, M F and McClain, W M and Hull, P G and Shi, Y and Quinby-Hunt, M S and Hearst, J E and Hunt, A J},
abstractNote = {The coupled-dipole approximation has been used to model polarized light-scattering data obtained from the sperm of the octopus [ital Eledone] [ital cirrhosa]. Mueller scattering-matrix elements (which describe how a sample alters the intensity and degree of polarization of scattered light) were measured as a function of angle. The sample was modeled as a helical fiber believed to correspond to a DNA protein complex. It was necessary to propose an inherent anisotropy in the polarizability of the fiber in order to fit the data. The direction of the principle axes of the polarizability were determined by comparing the model with experimental data. The results suggest that the 2-nm DNA fibers are perpendicular to the thick fiber that defines the helical geometry of the octopus sperm head.},
doi = {10.1364/AO.33.005733},
url = {https://www.osti.gov/biblio/6958288}, journal = {Applied Optics; (United States)},
issn = {0003-6935},
number = ,
volume = 33:24,
place = {United States},
year = {Sat Aug 20 00:00:00 EDT 1994},
month = {Sat Aug 20 00:00:00 EDT 1994}
}