A nuclear protein associated with human cancer cells binds preferentially to a human repetitive DNA sequence

Journal Article · Wed Nov 01 00:00:00 EST 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

OSTI ID:6957971

Gao, J ^[1]; Law, M L; Puck, T T ^[2]

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1. Eleanor Roosevelt Institute for Cancer Research, Denver, CO (USA)
2. Eleanor Roosevelt Institute for Cancer Research, Denver, CO (USA) Univ. of Colorado Health Sciences Center, Denver (USA)

A protein (Rp66) of 66 kDa was shown by DNA-binding protein blot assay to bind to a human repetitive DNA sequence (low-repeat sequences; LRS) in each of 10 transformed human cell lines examined. This protein-DNA interaction was not observed in 11 normal human cell cultures or in the Chinese hamster cell line CHO-K1. Gel retardation assay confirmed the specificity of the protein-DNA binding between Rp66 and LRS. In a histiocytic lymphoma human cell line, U937, that can be induced to differentiate in the presence of phorbol ester, this binding disappeared after cell differentiation. These together with other results cited suggest a regulatory role for these repetitive sequences in the human genome, with particular application to cancer.

• OSTI ID: 6957971
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 86:21; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
DNA
CROSS-LINKING
NUCLEOPROTEINS
TUMOR CELLS
CELL DIFFERENTIATION
AUTORADIOGRAPHY
CHO CELLS
ELECTROPHORESIS
FIBROBLASTS
LYMPHOMAS
PHOSPHORUS 32
PURIFICATION
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHEMICAL REACTIONS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DISEASES
IMMUNE SYSTEM DISEASES
ISOTOPES
LIGHT NUCLEI
NEOPLASMS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
POLYMERIZATION
PROTEINS
RADIOISOTOPES
SOMATIC CELLS
550201* - Biochemistry- Tracer Techniques