In vitro reversibility of cadmium-induced inhibition of phagocytosis
Cadmium chloride, administered chronically in the drinking water of CBA/H mice, produced a delayed clearance of particles and soluble material bearing Fc fragments. The inhibition was reversed upon removal of the cadmium from the water, even though a tissue load of cadmium persisted. An in vitro system was developed to analyze the mechanism of the inhibition. Binding and ingestion of sheep red blood cells (E) treated with IgG (for measurement of Fc receptor activity) or IgM and complement (C) (for measurement of complement receptor activity) were studied in resident and elicited murine peritoneal macrophages in vitro. Elicited macrophages provided the most definitive test system. With this system, there was significant inhibition of ingestion of E-IgG and E-IgMC. Binding of both types of erythrocytes was not inhibited, except at the highest concentration (10/sup -4/ M) of CdCl/sub 2/ at which binding of only E-IgMC was affected. These effects were reversible upon removal of cadmium. Migration of Fc and C receptors on the macrophage surface was not significantly affected by cadmium. In general, cadmium did not alter the expression or the binding of Fc or C receptors on macrophages. The mechanism for delayed clearance appears to be due to inhibited internalization of the particles. This is consistent with the interpretation that cadmium is a membrane active agent.
- Research Organization:
- Univ. of California, Los Angeles
- OSTI ID:
- 6956421
- Journal Information:
- Environ. Res.; (United States), Vol. 41:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CADMIUM
TOXICITY
MICE
PHAGOCYTOSIS
CADMIUM CHLORIDES
CLEARANCE
DRINKING WATER
ERYTHROCYTES
IN VITRO
INHIBITION
MACROPHAGES
RECEPTORS
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CADMIUM COMPOUNDS
CADMIUM HALIDES
CHLORIDES
CHLORINE COMPOUNDS
CONNECTIVE TISSUE CELLS
ELEMENTS
HALIDES
HALOGEN COMPOUNDS
HYDROGEN COMPOUNDS
MAMMALS
MATERIALS
MEMBRANE PROTEINS
METALS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PHAGOCYTES
PROTEINS
RODENTS
SOMATIC CELLS
VERTEBRATES
WATER
560300* - Chemicals Metabolism & Toxicology