Carbon uptake in a marine diatom during acute exposure to ultraviolet B radiation: Relative importance of damage and repair

Journal Article · Fri Apr 01 00:00:00 EST 1994 · Journal of Phycology; (United States)

DOI:https://doi.org/10.1111/j.0022-3646.1994.00183.x· OSTI ID:6954991

Lesser, M P ^[1]; Cullen, J J ^[2]; Neale, P J ^[3]

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1. Bigelow Lab. for Ocean Sciences, West Boothbay Harbor, ME (United States)
2. Bigelow Lab. for Ocean Sciences, West Boothbay Harbor, ME (United States) Dalhousie Univ., Halifax, Nova Scotia (Canada)
3. Bigelow Lab. for Ocean Sciences, West Boothbay Harbor, ME (United States) Univ. of California, Berkeley, CA (United States)

Experiments on a marine diatom, Thalassiosira pseudonana (Hustedt) clone 3H, demonstrate that under moderate photon flux densities (75 [mu]mol quanta [center dot] m[sup [minus]2][center dot]s[sup [minus]1]) of visible light inhibition of photosynthesis by supplemental ultraviolet (UV) radiation (UV-B: 280-320 nm) is well described as a hyperbolic function of UV-B irradiance for time scales of 0.5-4 h. Results are consistent with predictions of a recently developed model of photosynthesis under the influence of UV and visible irradiance. Although net destruction of chlorophyll occurs during a 4-h exposure to UV-B, and the effect is a function of exposure, the principal effect of UV-B is a decrease in chlorophyll-specific photosynthetic rate. The dependence of photoinhibition on dosage rate, rather than cumulative dose, and the hyperbolic shape of the relationship are consistent with net photoinhibition being an equilibrium between damage and repair. The ratio of damage to repair is estimated by a mathematical analysis of the inhibition of photosynthesis during exposures to UV-B. A nitrate-limited culture was much more sensitive to UV-B than were the nutrient-replete cultures, but the kinetics of photoinhibition were similar. The analysis suggests that the nutrient-limited culture was much more sensitive than the nutrient-replete cultures because repair or turnover of critical proteins associated with photosynthesis is inhibited. An inhibitor of chloroplast protein synthesis was used to suppress repair processes. Photoinhibition by UV-B was enhanced, and inhibition was a function of cumulative dose, as expected if damage were not countered by repair. The fundamental importance of repair processes should be considered in the design of field experiments and models of UV-B effects in the environment, especially in the context of vertical mixing. Repair processes must also be considered whenever biological weighting functions are developed. 69 refs., 6 figs., 3 tabs.

• OSTI ID: 6954991
• Journal Information: Journal of Phycology; (United States), Vol. 30:2; ISSN 0022-3646
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
DIATOMS
BIOLOGICAL ADAPTATION
BIOLOGICAL RADIATION EFFECTS
PHOTOSYNTHESIS
ULTRAVIOLET RADIATION
ALGAE
BIOLOGICAL EFFECTS
CHEMICAL REACTIONS
CHROMOPHYCOTA
ELECTROMAGNETIC RADIATION
PHOTOCHEMICAL REACTIONS
PLANTS
RADIATION EFFECTS
RADIATIONS
SYNTHESIS
560130* - Radiation Effects on Microorganisms