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Title: Separation of gonadotropic fractions with different species specificities from tuna pituitaries

Abstract

Eight different gonadotropic glycoprotein fractions were separated from the acetone-dried powder of yellow fin tuna pituitary glands by successive chromatographies on Superose 12 for gel filtration and Mono Q for anion exchange using the Pharmacia fast protein liquid chromatography system. This was preceded by preliminary separations using an ammonium sulfate precipitation method and affinity chromatography on concanavalin A-Sepharose. For biological characterization, we employed two radioreceptor assay systems, one using goby testis plasma membranes and silver carp GTH as the receptor and radioligand, respectively, and the other using testis plasma membranes of the yellow fin tuna and gonadotropin of the same species, respectively. We also employed two testicular cyclic AMP accumulation bioassay methods in vitro, one with the goby testis and the other with the mackerel testis. The least acidic fraction after Mono Q was further separated into four subfractions by rechromatography with Mono Q. They were strongly active in the tuna and mackerel assays but almost inactive in the goby assays. They were referred to as tuna-type tuna gonadotropin. In contrast, the most acidic fraction obtained after the first Mono Q was active in the goby assays but almost inactive in the tuna and mackerel assays. It was referred tomore » as goby-type tuna gonadotropin. The intermediate fractions were active on both assays and are considered to be mixtures of tuna-type and goby-type gonadotropins. The reason for the presence of gonadotropin inactive to homologous species is discussed from the evolutionary viewpoint.« less

Authors:
;
Publication Date:
Research Org.:
Waseda Univ., Tokyo (Japan)
OSTI Identifier:
6954429
Resource Type:
Journal Article
Journal Name:
Gen. Comp. Endocrinol.; (United States)
Additional Journal Information:
Journal Volume: 70:2
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; GONADOTROPINS; FRACTIONATION; AMP; ELECTROPHORESIS; FISHES; ION EXCHANGE CHROMATOGRAPHY; LIQUID COLUMN CHROMATOGRAPHY; PITUITARY GLAND; RADIORECEPTOR ASSAY; RECEPTORS; SPECIFICITY; TESTES; TUNA; ANIMALS; AQUATIC ORGANISMS; BODY; CHROMATOGRAPHY; ENDOCRINE GLANDS; GLANDS; GONADS; HORMONES; ISOTOPE APPLICATIONS; MALE GENITALS; MEMBRANE PROTEINS; NUCLEOTIDES; ORGANIC COMPOUNDS; ORGANS; PEPTIDE HORMONES; PITUITARY HORMONES; PROTEINS; SEPARATION PROCESSES; TRACER TECHNIQUES; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Ando, H, and Ishii, S. Separation of gonadotropic fractions with different species specificities from tuna pituitaries. United States: N. p., 1988. Web. doi:10.1016/0016-6480(88)90139-6.
Ando, H, & Ishii, S. Separation of gonadotropic fractions with different species specificities from tuna pituitaries. United States. https://doi.org/10.1016/0016-6480(88)90139-6
Ando, H, and Ishii, S. 1988. "Separation of gonadotropic fractions with different species specificities from tuna pituitaries". United States. https://doi.org/10.1016/0016-6480(88)90139-6.
@article{osti_6954429,
title = {Separation of gonadotropic fractions with different species specificities from tuna pituitaries},
author = {Ando, H and Ishii, S},
abstractNote = {Eight different gonadotropic glycoprotein fractions were separated from the acetone-dried powder of yellow fin tuna pituitary glands by successive chromatographies on Superose 12 for gel filtration and Mono Q for anion exchange using the Pharmacia fast protein liquid chromatography system. This was preceded by preliminary separations using an ammonium sulfate precipitation method and affinity chromatography on concanavalin A-Sepharose. For biological characterization, we employed two radioreceptor assay systems, one using goby testis plasma membranes and silver carp GTH as the receptor and radioligand, respectively, and the other using testis plasma membranes of the yellow fin tuna and gonadotropin of the same species, respectively. We also employed two testicular cyclic AMP accumulation bioassay methods in vitro, one with the goby testis and the other with the mackerel testis. The least acidic fraction after Mono Q was further separated into four subfractions by rechromatography with Mono Q. They were strongly active in the tuna and mackerel assays but almost inactive in the goby assays. They were referred to as tuna-type tuna gonadotropin. In contrast, the most acidic fraction obtained after the first Mono Q was active in the goby assays but almost inactive in the tuna and mackerel assays. It was referred to as goby-type tuna gonadotropin. The intermediate fractions were active on both assays and are considered to be mixtures of tuna-type and goby-type gonadotropins. The reason for the presence of gonadotropin inactive to homologous species is discussed from the evolutionary viewpoint.},
doi = {10.1016/0016-6480(88)90139-6},
url = {https://www.osti.gov/biblio/6954429}, journal = {Gen. Comp. Endocrinol.; (United States)},
number = ,
volume = 70:2,
place = {United States},
year = {Sun May 01 00:00:00 EDT 1988},
month = {Sun May 01 00:00:00 EDT 1988}
}