The effect of aging on mitochondrial proteins in germinating soybean embryonic axes

Miscellaneous · Fri Jan 01 00:00:00 EST 1988

OSTI ID:6948583

Furman, K C

Aging-induced deterioration is a major problem associated with seed storage. Impairment of mitochondrial function is one of the first effects of aging. The composition and synthesis of nuclear and mito-coded mitochondrial proteins from soybean (Glycine max. L. Merr.) embryonic axes were studied to elucidate the cause of impaired respiratory development during germination of aged seeds. Axes excised from high vigor (HV) seeds and aged or low vigor (LV) seeds were protected from imbibition injury and germinated for various times, or excised from developing seeds, and then radiolabeled for one hour in ({sup 35}S)methionine. Mitochondria were then isolated and total mitochondrial protein was subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), followed by quantitative staining and fluorography of labeled polypeptides. Alternatively, an original two-dimensional native-to-denaturing gel electrophoretic technique was used to analyze native protein associations and to purify a 23 kD polypeptide.

• Research Organization: Maryland Univ., College Park, MD (USA)
• OSTI ID: 6948583
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
POLYPEPTIDES
BIOSYNTHESIS
SEEDS
STORAGE LIFE
SOYBEANS
ONTOGENESIS
AGE DEPENDENCE
CHEMICAL COMPOSITION
ELECTROPHORESIS
GERMINATION
GLYCINE HISPIDA
METHIONINE
MITOCHONDRIA
SULFUR 35
TRACER TECHNIQUES
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
DRUGS
EVEN-ODD NUCLEI
FOOD
ISOTOPE APPLICATIONS
ISOTOPES
LEGUMINOSAE
LIGHT NUCLEI
LIPOTROPIC FACTORS
MAGNOLIOPHYTA
MAGNOLIOPSIDA
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANOIDS
PEPTIDES
PLANTS
PROTEINS
RADIOISOTOPES
SULFUR ISOTOPES
SYNTHESIS
VEGETABLES
550201* - Biochemistry- Tracer Techniques