Cholinergic neurotransmission in human corpus cavernosum. II. Acetylcholine synthesis

Journal Article · Tue Mar 01 00:00:00 EST 1988 · American Journal of Physiology; (USA)

OSTI ID:6945492

Blanco, R; De Tejada, S; Goldstein, I; Krane, R J; Wotiz, H H; Cohen, R A ^[1]

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1. Boston Univ. Medical Center, MA (USA)

Physiological and histochemical evidence indicates that cholinergic nerves may participate in mediating penile erection. Acetylcholine synthesis and release was studied in isolated human corporal tissue. Human corpus cavernosum incubated with ({sup 3}H)choline accumulated ({sup 3}H)choline and synthesized ({sup 3}H)acethylcholine in an concentration-dependent manner. ({sup 3}H)Acetylcholine accumulation by the tissue was inhibited by hemicholinium-3, a specific antagonist of the high-affinity choline transport in cholinergic nerves. Transmural electrical field stimulation caused release of ({sup 3}H)acetylcholine which was significantly diminished by inhibiting neurotransmission with calcium-free physiological salt solution or tetrodotoxin. These observations provide biochemical and physiological evidence for the existence of cholinergic innervation in human corpus cavernosum.

• OSTI ID: 6945492
• Journal Information: American Journal of Physiology; (USA), Vol. 254:3; ISSN 0002-9513
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ACETYLCHOLINE
BIOSYNTHESIS
CHOLINE
UPTAKE
MALE GENITALS
PHYSIOLOGY
TRITIUM COMPOUNDS
BIOELECTRICITY
BIOPSY
MEN
NERVE CELLS
NEUROREGULATORS
TRANSFERASES
ALCOHOLS
AMINES
AMMONIUM COMPOUNDS
ANIMAL CELLS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BODY
DIAGNOSTIC TECHNIQUES
DRUGS
ELECTRICITY
ENZYMES
ESTERS
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
LIPOTROPIC FACTORS
MALES
MAMMALS
MAN
ORGANIC COMPOUNDS
ORGANS
PARASYMPATHOMIMETICS
PRIMATES
QUATERNARY COMPOUNDS
SOMATIC CELLS
SYNTHESIS
VERTEBRATES
551001* - Physiological Systems- Tracer Techniques