TeBG- and CBG-bound steroid hormones in rabbits are available for influx into uterus in vivo

Journal Article · Fri Jan 01 00:00:00 EST 1988 · American Journal of Physiology; (USA)

OSTI ID:6945403

Chaudhuri, G; Steingold, K A; Pardridge, W M; Judd, H L ^[1]

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1. Univ. of California, Los Angeles (USA)

The metabolic clearance rate (MCR) of gonadal or adrenal steroid hormones in rabbits often does not bear the expected inverse relationship with hormone binding to testosterone-binding globulin (TeBG) or corticosteroid-binding globulin (CBG). This suggests TeBG or CBG may not impede steroid hormone delivery to tissues. The effects of rabbit plasma proteins on the influxes of {sup 3}H-labeled steroids from the circulation into the rabbit uterus were measured in vivo using a tissue sampling single-injection technique. In the absence of plasma proteins, estradiol (E{sub 2}) and testosterone (T) were freely diffusible through the uterine microvasculature (i.e., extraction >80%). The extractions of dihydrostestosterone (DHT) and corticosterone (B) ranged from 60 to 72%, while that of cortisol (F) was reduced at 40%. Rabbit serum exerted no inhibition of the influxes of the steroids tested. The influxes of T and B greatly exceeded the rates that would be expected if only the free and albumin-bound fractions estimated in vitro were diffusible in vivo. However, the extraction of ({sup 3}H)corticosteroid-binding globulin or bovine ({sup 3}H)albumin were low, consistent with little, if any, extravascular uptake of the plasma proteins. The results indicate both albumin-bound and globulin-bound steroid hormone are available for transport into the uterus in the rabbit in vivo without significant exodus of the plasma protein, per se.

• OSTI ID: 6945403
• Journal Information: American Journal of Physiology; (USA), Vol. 254:1; ISSN 0002-9513
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
STEROID HORMONES
BLOOD-PLASMA CLEARANCE
TRITIUM COMPOUNDS
UPTAKE
ALBUMINS
CORTICOSTEROIDS
GLOBULINS
PROTEINS
RABBITS
TESTOSTERONE
UTERUS
ADRENAL HORMONES
ANDROGENS
ANDROSTANES
ANIMALS
BODY
CLEARANCE
FEMALE GENITALS
HORMONES
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
KETONES
MAMMALS
ORGANIC COMPOUNDS
ORGANS
PREGNANES
STEROIDS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques