Detection of biological warfare agents using the polymerase chain reaction. Final report, June-August 1991

Technical Report · Tue Sep 01 00:00:00 EDT 1992

OSTI ID:6943738

Mann, B J

The detection of biological warfare agents is an important mission for the U.S. Army. This report explores the feasibility of using the polymerase chain reaction as a means of rapid detection of biological warfare agents. Two levels of detection are proposed. The first level is group specific detection, using primers derived from 16S rDNA sequences, to detect various groups of pathogenic bacteria. The second level is species-specific detection using primers derived from DNA sequences, unique to each pathogenic organism targeted for detection. Specific examples of Vibrio cholerae, Francisella tularensis, Yersinia pestis, Staphylococcus aureus, and Bacillus anthracis are described.

• Research Organization: Battelle Columbus Labs., Research Triangle Park, NC (United States)
• OSTI ID: 6943738
• Report Number(s): AD-A-259391/1/XAB; CNN: DAAL03-86-D-0001
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
45 MILITARY TECHNOLOGY, WEAPONRY, AND NATIONAL DEFENSE
BIOLOGICAL WARFARE AGENTS
DETECTION
BACILLUS
BACTERIA
BIOASSAY
BIOLOGICAL WARFARE
DNA
FEASIBILITY STUDIES
MICROORGANISMS
POLYMERIZATION
STAPHYLOCOCCUS
US DOD
USA
CHEMICAL REACTIONS
DEVELOPED COUNTRIES
NATIONAL ORGANIZATIONS
NORTH AMERICA
NUCLEIC ACIDS
ORGANIC COMPOUNDS
US ORGANIZATIONS
WARFARE
WEAPONS
560300* - Chemicals Metabolism & Toxicology
450600 - Military Technology
Weaponry
& National Defense- Chemical & Biological- (1990)