Comparison of bile acid synthesis determined by isotope dilution versus fecal acidic sterol output in human subjects

Journal Article · Sat May 01 00:00:00 EDT 1982 · Lipids; (United States)

DOI:https://doi.org/10.1007/BF02535192· OSTI ID:6943558

Duane, W C; Holloway, D E; Hutton, S W; Corcoran, P J; Haas, N A

Fecal acidic sterol output has been found to be much lower than bile acid synthesis determined by isotope dilution. Because of this confusing discrepancy, we compared these 2 measurements done simultaneously on 13 occasions in 5 normal volunteers. In contrast to previous findings, bile acid synthesis by the Lindstedt isotope dilution method averaged 16.3% lower than synthesis simultaneously determined by fecal acidic sterol output (95% confidence limit for the difference - 22.2 to -10.4%). When one-sample determinations of bile acid pools were substituted for Lindstedt pools, bile acid synthesis by isotope dilution averaged 5.6% higher than synthesis by fecal acidic sterol output (95% confidence limits -4.9 to 16.1%). These data indicate that the 2 methods yield values in reasonably close agreement with one another. If anything, fecal acidic sterol outputs are slightly higher than synthesis by isotope dilution.

• Research Organization: Department of Medicine, Veterans Administration Medical Center, Minneapolis
• OSTI ID: 6943558
• Journal Information: Lipids; (United States), Vol. 17:5
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BILE ACIDS
BIOSYNTHESIS
RADIOISOTOPES
ISOTOPE DILUTION
COMPARATIVE EVALUATIONS
FECES
PATIENTS
QUANTITATIVE CHEMICAL ANALYSIS
STEROLS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
CARBOXYLIC ACIDS
CHEMICAL ANALYSIS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
MATERIALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
STEROIDS
SYNTHESIS
TRACER TECHNIQUES
WASTES
550201* - Biochemistry- Tracer Techniques
551001 - Physiological Systems- Tracer Techniques