Hypervirulence of Agrobacterium tumefaciens A281 is encoded in a region of pTiBo542 outside of T-DNA

Journal Article · Mon Dec 01 00:00:00 EST 1986 · J. Bacteriol.; (United States)

OSTI ID:6942563

Hood, E E; Helmer, G L; Fraley, R T; Chilton, M D

A binary-vectory strategy was used to study the hypervirulence of Agrobacterium tumefaciens A281, an L,L-succinamopine strain. Strain A281 is hypervirulent on several solanaceous plants. Plasmids were constructed (pCS65 and pCS277) carrying either the transferred DNA (T-DNA) or the remainder of the tumor-inducing (Ti) plasmid (pEHA101) from this strain and tested each of these constructs were tested in trans with complementary each of regions from heterologous Ti plasmids. Hypervirulence on tobacco could be reconstructed in a bipartite strain with the L,L-succinamopine T-DNA and the vir region on separate plasmids. pEHA101 was able to complement octopine T-DNA to hypervirulence on tobacco and tomato plants. Nopaline T-DNA was complemented better on tomato plants by pEHA101 than it was by its own nopaline vir region, but not to hypervirulence. L,L-Succinamopine T-DNA could not be complemented to hypervirulence on tobacco and tomato plants with either heterologous vir region. From these results the authors suggest that the hypervirulence of strain A281 is due to non-T-DNA sequences on the Ti plasmid.

• Research Organization: Washington Univ., St. Louis, MO
• DOE Contract Number: AC02-81ER10888
• OSTI ID: 6942563
• Journal Information: J. Bacteriol.; (United States), Vol. 168:3
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BACTERIA
VIRULENCE
ONCOGENIC TRANSFORMATIONS
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SOLANUM
DNA
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PLANT DISEASES
PLANTS
PLASMIDS
RECOMBINANT DNA
TOMATOES
CELL CONSTITUENTS
CELL TRANSFORMATIONS
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