Nuclear magnetic resonance studies of the blue copper protein plastocyanin
Plastocyanins (pcy) from a higher plant (spinach, Spinacia oleracea), a cyanobacterium (Anabaena variabilis), and a green alga (Chlorella fusca), were studied by /sup 1/H and /sup 13/C NMR. All three histidine /sup 13/C/sub ..gamma../ peaks in A. variabilis pcy were assigned. Previous assignments of the homologous /sup 13/C/sub ..gamma../ peaks in spinach pcy were corrected. A glutamic acid /sup 13/C/sub delta/ peak was found to titrate with an unusually high pK/sub a/ (approx.5.3); this peak was assigned to the N-terminal Glu-(-2). Exchange of /sup 113/Cd(II) for Cu(I) led to changes in chemical shifts of groups at the metal binding site. Spectral changes observed with residues as far as 18 A from the metal site indicate that the structure at the metal binding site is coupled to the structure at the opposite end of the molecule.
- Research Organization:
- Purdue Univ., Lafayette, IN (USA)
- OSTI ID:
- 6942538
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
METALLOPROTEINS
MOLECULAR STRUCTURE
CARBON 13
CHLORELLA
COPPER
CYANOBACTERIA
HISTIDINE
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
NUCLEAR MAGNETIC RESONANCE
SPINACH
ALGAE
AMINO ACIDS
AZOLES
CARBON ISOTOPES
CARBOXYLIC ACIDS
ELEMENTS
EVEN-ODD NUCLEI
FOOD
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
IMIDAZOLES
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
METALS
MICROORGANISMS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PLANTS
PROTEINS
RESONANCE
STABLE ISOTOPES
TRANSITION ELEMENTS
UNICELLULAR ALGAE
VEGETABLES
550301* - Cytology- Tracer Techniques