Site-specific aflatoxin B sub 1 adduction of sequence-positioned nucleosome core particles

Miscellaneous · Fri Jan 01 00:00:00 EST 1988

OSTI ID:6940418

Moyer, R A

The question of how the presence of nucleosomal packing of DNA modifies carcinogen interaction at specific sites cannot be answered by studies on whole chromatin or bulk nucleosomes because of the heterogeneity of DNA sequences in the particles. This problem was circumvented by constructing nucleosomes that are homogenous in DNA-histone contact points. A cloned DNA fragment, containing a sea urchin 5S gene which precisely positions a histone octamer was employed. By using {sup 32}P end-labeled DNA and genotoxins that allow cleavage at sites of attack, the frequency of adduction at every susceptible nucleotide can be determined on sequencing gels. The small methylating agent dimethyl sulfate (DMS) and the bulky alkylating agent afatoxin B{sub 1}-dichloride (AFB{sub 1}-Cl{sub 2}) were used to probe the influence of DNA-histone interactions on DNA alkylation patterns in sequence-positioned core particles.

• Research Organization: Oregon State Univ., Corvallis, OR (USA)
• OSTI ID: 6940418
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
AFLATOXIN
BIOCHEMICAL REACTION KINETICS
HISTONES
GENES
ALKYLATION
CARCINOGENS
DNA SEQUENCING
DNA-CLONING
NUCLEOSOMES
PHOSPHORUS 32
RECEPTORS
SEA URCHINS
TRACER TECHNIQUES
ANIMALS
AQUATIC ORGANISMS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHEMICAL REACTIONS
CHROMATIN
CLONING
DAYS LIVING RADIOISOTOPES
DNA HYBRIDIZATION
ECHINODERMS
HYBRIDIZATION
INVERTEBRATES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
MEMBRANE PROTEINS
NUCLEI
NUCLEOPROTEINS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
STRUCTURAL CHEMICAL ANALYSIS
550201* - Biochemistry- Tracer Techniques