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Title: Regulation of protein synthesis during sea urchin early development

Abstract

Fertilization of the sea urchin egg results in a 20-40 fold increase in the rate of protein synthesis. The masked message hypothesis proposes that mRNAs are masked or unavailable for translation in the egg. We devised an in vivo assay to test this hypothesis. Our results show that masked mRNAs limit protein synthesis in the unfertilized egg. In addition, we show that protein synthesis is also regulated at the level of translational machinery. Following fertilization is a period of rapid cell divisions. This period, known as the rapid cleavage stage, is characterized by the transient synthesis of a novel set of proteins. The synthesis of these proteins is programmed by maternal mRNAs stored in the unfertilized egg. To study the behavior of these mRNAs, we prepared a cDNA library from polysomal poly (A+) RNA from 2-hour embryos. ({sup 32}P) labeled probes, prepared from the cDNA library, were used to monitor the levels of individual mRNAs in polysomes at fertilization and during early development.

Authors:
Publication Date:
Research Org.:
Texas Univ., Austin, TX (USA)
OSTI Identifier:
6939602
Resource Type:
Miscellaneous
Resource Relation:
Other Information: Thesis (Ph. D.)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; MESSENGER-RNA; TRANSCRIPTION; ONTOGENESIS; BIOCHEMISTRY; PROTEINS; BIOSYNTHESIS; BIOASSAY; CELL DIFFERENTIATION; CELL DIVISION; EMBRYOS; PHOSPHORUS 32; POST-TRANSLATION MODIFICATION; SEA URCHINS; TRACER TECHNIQUES; ANIMALS; AQUATIC ORGANISMS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CHEMISTRY; DAYS LIVING RADIOISOTOPES; ECHINODERMS; INVERTEBRATES; ISOTOPE APPLICATIONS; ISOTOPES; LIGHT NUCLEI; NUCLEI; NUCLEIC ACIDS; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; PHOSPHORUS ISOTOPES; RADIOISOTOPES; RNA; SYNTHESIS; 550201* - Biochemistry- Tracer Techniques; 550801 - Morphology- Tracer Techniques

Citation Formats

Kelso, L C. Regulation of protein synthesis during sea urchin early development. United States: N. p., 1989. Web.
Kelso, L C. Regulation of protein synthesis during sea urchin early development. United States.
Kelso, L C. 1989. "Regulation of protein synthesis during sea urchin early development". United States.
@article{osti_6939602,
title = {Regulation of protein synthesis during sea urchin early development},
author = {Kelso, L C},
abstractNote = {Fertilization of the sea urchin egg results in a 20-40 fold increase in the rate of protein synthesis. The masked message hypothesis proposes that mRNAs are masked or unavailable for translation in the egg. We devised an in vivo assay to test this hypothesis. Our results show that masked mRNAs limit protein synthesis in the unfertilized egg. In addition, we show that protein synthesis is also regulated at the level of translational machinery. Following fertilization is a period of rapid cell divisions. This period, known as the rapid cleavage stage, is characterized by the transient synthesis of a novel set of proteins. The synthesis of these proteins is programmed by maternal mRNAs stored in the unfertilized egg. To study the behavior of these mRNAs, we prepared a cDNA library from polysomal poly (A+) RNA from 2-hour embryos. ({sup 32}P) labeled probes, prepared from the cDNA library, were used to monitor the levels of individual mRNAs in polysomes at fertilization and during early development.},
doi = {},
url = {https://www.osti.gov/biblio/6939602}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Sun Jan 01 00:00:00 EST 1989},
month = {Sun Jan 01 00:00:00 EST 1989}
}

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