Enhanced binding of /sup 3/H-arginine8-vasopressin in the Brattleboro rat
Specific binding sites for 3(H)-arginine8-vasopressin (AVP) were characterized using membrane preparations of liver, renal medulla and brain (septal) tissue of heterozygous (HE) and homozygous (HO) Brattleboro (BB) rats. Measurement of binding sites indicated that significantly greater numbers of AVP receptors are present in the liver and septum of HO-BB rats. Similar numbers of AVP receptors were present in renal medullary tissue from HO-BB and HE-BB rats. Higher equilibrium dissociation constants were measured in the HO-BB septal tissue indicating a lower affinity of the brain receptor for 3(H)-AVP than in heterozygotes. No significant differences in AVP receptor affinity were noted in liver or kidney tissue. It is concluded that up-regulation of AVP receptor number and, in the brain, alterations in AVP receptor affinity may occur in the absence of endogenous AVP.
- Research Organization:
- Veterans Administration Medical Center, Seattle, WA
- OSTI ID:
- 6937214
- Journal Information:
- Peptides (Fayetteville, N.Y.); (United States), Vol. 4
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
VASOPRESSIN
RECEPTORS
ARGININE
BRAIN
KIDNEYS
LIVER
RADIORECEPTOR ASSAY
RATS
TRITIUM COMPOUNDS
AMINO ACIDS
ANIMALS
BODY
CARBOXYLIC ACIDS
CENTRAL NERVOUS SYSTEM
DIGESTIVE SYSTEM
GLANDS
HORMONES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
NERVOUS SYSTEM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PITUITARY HORMONES
PROTEINS
RODENTS
TRACER TECHNIQUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques