Monoclonal antibodies to gonadotropin subunits

Journal Article · Tue Jan 01 00:00:00 EST 1985 · Methods Enzymol.; (United States)

DOI:https://doi.org/10.1016/0076-6879(85)09120-0· OSTI ID:6936396

Ehrlich, P H; Moyle, W R; Canfield, R E

The production of monoclonal antibodies to peptide hormones, with their unifocal binding sites, can provide tools for understanding hormone structure and function. The paper focuses on techniques that are important for the study of monoclonal antibodies to chorionic gonadotropin (hCG), including hybridoma production, methods of screening for desired clones, properties of the monoclonal antibodies, effect of antibodies on hormone-receptor interaction, inhibition of binding of radiolabeled hCG, inhibition of hCG induced steroidogenesis, determination of relative orientation of epitopes, and synergistic actions of monoclonal antibodies to hCG.

• OSTI ID: 6936396
• Journal Information: Methods Enzymol.; (United States), Vol. 109
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
HCG
MONOCLONAL ANTIBODIES
BIOCHEMICAL REACTION KINETICS
CLONE CELLS
EXPERIMENTAL DATA
HYBRIDOMAS
IODINE 125
LABELLED COMPOUNDS
RECEPTORS
SYNERGISM
TRACER TECHNIQUES
ANIMAL CELLS
ANTIBODIES
BETA DECAY RADIOISOTOPES
CELL CULTURES
DATA
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
GONADOTROPINS
HORMONES
INFORMATION
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MEMBRANE PROTEINS
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PITUITARY HORMONES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
550201* - Biochemistry- Tracer Techniques