Similarities between human eosinophil-derived neurotoxin and eosinophil cationic protein: homology with ribonuclease
The eosinophil granule contains a series of basic proteins, including eosinophil-derived neurotoxin (EDN) and eosinophil cationic protein (ECP). EDN and ECP were isolated from eosinophil granules by heparin-Sepharose chromatography. Radioimmunoassay of fractions from heparin-Sepharose showed one peak of EDN activity and two peaks of ECP activity (termed ECP-1 and ECP-2). EDN, ECP-1, and ECP-2 each exhibited heterogeneity in charge and molecular weight when analyzed by two-dimensional electrophoresis. Digestion of EDN and both ECP's with endoglycosidase-F decreased their molecular weights indicating that their heterogeneity is due in part to n-linked oligosaccharides. Amino acid sequence analyses showed that ECP-1 and ECP-2 were identical from residues 1 through 59 and that EDN and ECP sequences were highly homologous (37 of 55 residues identical). Both EDN and ECP NH/sub 2/-terminal sequences showed significant homology to RNase, especially in regions of the RNase molecule involved in ligand binding. EDN, ECP-1 and ECP-2 had neurotoxic activity, causing the Gordon phenomenon at doses down 0.15 microgram; the proteins were comparable in their activities. These results indicate that EDN and ECP are related proteins and suggest that they derived from genes associated with the RNase family.
- Research Organization:
- Mayo Clinic and Foundation, Rochester, MN
- OSTI ID:
- 6936237
- Report Number(s):
- CONF-8604222-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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PROTEINS
AMINO ACID SEQUENCE
RADIOIMMUNOASSAY
CHROMATOGRAPHY
COMPARATIVE EVALUATIONS
ELECTROPHORESIS
MAN
MOLECULAR WEIGHT
RNA-ASE
TOXINS
ANIMALS
ANTIGENS
ENZYMES
ESTERASES
HYDROLASES
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
MAMMALS
MATERIALS
MOLECULAR STRUCTURE
ORGANIC COMPOUNDS
PHOSPHODIESTERASES
PRIMATES
RADIOASSAY
RADIOIMMUNOLOGY
SEPARATION PROCESSES
TOXIC MATERIALS
TRACER TECHNIQUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques