Phorbol myristate acetate and dioctanoylglycerol inhibit transport in rabbit proximal convoluted tubule
- Univ. of Texas Health Science Center, Dallas (USA)
The present in vitro microperfusion study examined the effect of protein kinase C activation on transport in the rabbit proximal convoluted tubule (PCT). PCT were perfused with an ultrafiltrate-like solution and were bathed in a serumlike albumin solution. Addition of phorbol 12-myristate 13-acetate, an activator of protein kinase C, inhibited volume absorption from 1.06 {plus minus} 0.10 to 0.77 {plus minus} 0.07 nl{center dot}mm{sup {minus}1}min{sup {minus}1}, and 0.76 {plus minus} 0.14 to 0.48 {plus minus} 0.08 nl{center dot}mm{sup {minus}1}{center dot}min{sup {minus}1}, respectively. Bath phorbol 12-myristate 13-acetate had no effect on volume absorption. In contrast, bath 4{alpha}-phorbol, an inactive phorbol that does not activate protein kinase C, had no effect on J{sub v}. Bath L-{alpha}-dioctanoylglycerol, another known activator of protein kinase C, inhibited volume absorption. A 10-fold lower concentration of L-{alpha}-dioctanoylglycerol had no effect on J{sub v}. Both 5 x 10{sup {minus}8} M phorbol 12-myristate 13-acetate and 10{sup {minus}4} M L-{alpha}-dioctanoylglycerol inhibited glucose, bicarbonate, and chloride transport in the PCT. These data are consistent with protein kinase C activation playing a role in the modulation of proximal tubular transport.
- OSTI ID:
- 6935966
- Journal Information:
- American Journal of Physiology; (USA), Vol. 254:1; ISSN 0002-9513
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ACID CARBONATES
MEMBRANE TRANSPORT
GLUCOSE
GLYCEROL
BIOLOGICAL EFFECTS
PHORBOL ESTERS
CARBON 14 COMPOUNDS
IN VITRO
INHIBITION
INULIN
PERFUSED TISSUES
PHOSPHOTRANSFERASES
RABBITS
TRITIUM COMPOUNDS
VOLUME
ALCOHOLS
ALDEHYDES
ANIMAL TISSUES
ANIMALS
BODY
CARBOHYDRATES
CARCINOGENS
ENZYMES
ESTERS
HEXOSES
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
LABELLED COMPOUNDS
MAMMALS
MONOSACCHARIDES
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
POLYSACCHARIDES
SACCHARIDES
TISSUES
TRANSFERASES
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology
551001 - Physiological Systems- Tracer Techniques