Effect of cell passage on the susceptibility of BALB/3T3 clone A31-1-1 cells to 3-methylcholanthrene-induced morphological transformation
The response of BALB/3T3 clone A31-1-1 cells to chemically induced morphological transformation was evaluated using 3-methylcholanthrene (MCA). Stock cultures were initiated from cryopreserved cells, grown in T25 flasks containing 5 ml of medium, and replated at subconfluency. Serially transferred cells were then subjected to transformation assay. After 24-hr seeding, cells were incubated 48 hr with MCA in a 5% CO/sub 2/ incubator. They were then rinsed and incubated for an additional 4 weeks with twice weekly medium change. Type III foci were scored after fixation and staining with Giemsa. With serial passage from the frozen state, cells of passages 3-14 had a low level of spontaneous transformation; zero to 6 type III foci per 20 dishes were counted. In the MCA-treated cultures the number of transformed foci, however, increased with passage. Such passage-related sensitivity to MCA was demonstrated for cells cultured in two batches of sera: one from MA Bioproducts (Lot no. 2E052) and the other from Armour Pharmaceuticals (Lot no. Y65801). The passage-related increase in number of transformed foci was not related to doubling time, cloning efficiency, or MCA-induced growth inhibition.
- Research Organization:
- Food and Drug Administration, Washington, DC
- OSTI ID:
- 6934699
- Journal Information:
- Environ. Mutagen.; (United States), Vol. 9:1
- Country of Publication:
- United States
- Language:
- English
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3-METHYLCHOLANTHRENE
BIOLOGICAL EFFECTS
FIBROBLASTS
ONCOGENIC TRANSFORMATIONS
CELL CULTURES
EMBRYONIC CELLS
MICE
MORPHOLOGICAL CHANGES
ANIMAL CELLS
ANIMALS
AROMATICS
CELL TRANSFORMATIONS
CONNECTIVE TISSUE CELLS
HYDROCARBONS
MAMMALS
ORGANIC COMPOUNDS
POLYCYCLIC AROMATIC HYDROCARBONS
RODENTS
SOMATIC CELLS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology