Leukocyte labeling in plasma made simple and efficient: Preparation and evaluation
Over the past few years the use of In-111 leukocytes (In-111-L) for abscess localization has become increasingly popular. Using less toxic Mercaptopyridine-N-oxide or its Na-salt (Merc, LD/sub 50/ 340 mg/kg b.w.) rather than oxine (LD/sub 50/ 88.8 mg/kg b.w.), In-111-L could be prepared either in salt balance media or preferably by a kit procedure in plasma. In the plasma preparation L concentrated in 0.5 ml plasma are added to 25 ..mu..g dry Merc and subsequently incubated at an ambient temperature for 20 minutes with a weak chelate of In-111 either dry or in solution. Greater than 90/sup 0/ labeling efficiency is achieved which compared favorably to 10 ..mu..g and 50 ..mu..g each of oxine and tropolone. Phagocytic ability of 10 million L exposed to up to 200 ..mu..g Merc remained unaffected. In-111-L and Ga-67 citrate were given to abscess bearing dogs and abscess/blood (24 hr.) ratios of 75 and 12 respectively were achieved. Those were similar to those obtained with In-111-oxine labeled L (75.2 vs. 75.8). Abscess/liver and abscess/spleen ratios obtained with In-111-merc labeled L (14.6 and 4.2 respectively) were more favorable than those obtained with In-111-oxine labeled L (4.2d and 1.8 respectively). These indicated that less quantity of L labeled with In-111-Merc was deposited in these organs. Furthermore, L labeled with In-111-Merc cleared more rapidly from human lungs than L labeled with In-111-oxine. Data indicate that use of Merc permits efficient L labeling in plasma, provides a kit method, and preserves L physiologic function.
- Research Organization:
- Thomas Jefferson Univ., Philadelphia, PA
- OSTI ID:
- 6933916
- Report Number(s):
- CONF-840619-; TRN: 87-008763
- Journal Information:
- J. Nucl. Med.; (United States), Vol. 25:5; Conference: 31. annual meeting of the Society of Nuclear Medicine, Los Angeles, CA, USA, 5 Jun 1984
- Country of Publication:
- United States
- Language:
- English
Similar Records
Simplified and efficient labeling of human platelets in plasma using indium-111-2-mercaptopyridine-N-oxide: preparation and evaluation
Evaluation of indium-111-2-mercaptopyridine-N-oxide for labeling leukocytes in plasma: a kit preparation
Related Subjects
INDIUM 111
DIAGNOSTIC USES
LEUKOCYTES
LABELLING
BLOOD PLASMA
CHEMICAL PREPARATION
DOGS
GALLIUM 67
HUMAN POPULATIONS
LIVER
LUNGS
OXINE
PHYSIOLOGY
PYRIDINE
SPLEEN
TRACER TECHNIQUES
ANIMALS
AROMATICS
AZAARENES
AZINES
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ELECTRON CAPTURE RADIOISOTOPES
GALLIUM ISOTOPES
GLANDS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MATERIALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
POPULATIONS
PYRIDINES
QUINOLINES
RADIOISOTOPES
RESPIRATORY SYSTEM
SYNTHESIS
USES
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics