Radiolabeling of infective larvae of Haemonchus contortus (Nematoda: Trichostrongyloidea) with /sup 75/Se-methionine and their performance as tracers in sheep
Haemonchus contortus infective larvae incorporated between 5 and 12 pCi/larva for each muCi of /sup 75/Se-methionine added per gram of fecal sediment. Thorough admixture of /sup 75/Se-methionine and fecal sediment was necessary to obtain approximately normal distribution and low variance of individual larval radioactivities. Ecdysis induced by treatment with 0.025% HClO in vitro resulted in loss of approximately 40% of the /sup 75/Se label of infective larvae. Loss of /sup 75/Se by parasitic larvae and adult H. contortus in vivo conformed to a two-component negative exponential function with half lives of 3.1 and 56 days acting on compartments representing 90% and 10%, respectively, of the /sup 75/Se label remaining after ecdysis. Labeled and unlabeled worms were readily distinguished by autoradiography 37 days after infection. No effect of gamma radiation arising from decay of /sup 75/Se in the range 130 to 1,300 pCi/larva could be measured in terms of survival or sex ratio of worms recovered at 17 days PI.
- Research Organization:
- Pastoral Research Laboratory, CSIRO, Armidale, N.S.W., Australia
- OSTI ID:
- 6929486
- Journal Information:
- J. Parasitol.; (United States), Vol. 69:5
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
LARVAE
LABELLING
AUTORADIOGRAPHY
METHIONINE
NEMATODES
SELENIUM 75
SHEEP
TRACER TECHNIQUES
AMINO ACIDS
ANIMALS
ASCHELMINTHES
BETA DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
HELMINTHS
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LIPOTROPIC FACTORS
MAMMALS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
RADIOISOTOPES
RUMINANTS
SELENIUM ISOTOPES
VERTEBRATES
550901* - Pathology- Tracer Techniques