Phagocytosis and transforming activity of crystalline metal sulfide particles are related to their negative surface charge

Journal Article · Fri Jan 01 00:00:00 EST 1982 · Carcinogenesis (N.Y.); (United States)

DOI:https://doi.org/10.1093/carcin/3.2.175· OSTI ID:6928523

Abbracchio, M P; Heck, J D; Costa, M

Crystalline nickel sulfide (alpha NiS) and cobalt sulfide (CoS2) particles can cause greater cell transformation and cellular toxicity than the respective amorphous metal sulfide particles. Cultured mammalian cells phagocytose the crystalline metal sulfide particles more readily than the amorphous ones. In the case of the nickel sulfides, the crystalline metal sulfide particles had negatively charged surfaces (Zeta potential: -27.012 mV) in contrast to the amorphous particles, which were positively charge (Zeta potential: +9.174 mV). X-ray photoelectron spectroscopy analysis of amorphous and crystalline NiS particles revealed that the outermost surface (1-4 nm) of the two particles had striking differences in Ni/S ratios and in their sulfur oxidation states. Rendering particles' surfaces more negative by reduction with lithium aluminum hydride enhanced their phagocytosis, and in the case of amorphous NiS chemical reduction resulted in an incidence of morphological transformation of Syrian hamster embryo cells comparable to that observed with untreated crystalline alpha NiS.

• Research Organization: University of Texas Medical School at Houston
• OSTI ID: 6928523
• Journal Information: Carcinogenesis (N.Y.); (United States), Vol. 3:2
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
COBALT SULFIDES
BIOLOGICAL EFFECTS
NICKEL SULFIDES
SURFACE ENERGY
MEASURING METHODS
CELL CULTURES
COMPARATIVE EVALUATIONS
ELECTRON MICROSCOPY
MAMMALS
OXIDATION
PARTICLE SIZE
PHAGOCYTOSIS
SENSITIVITY
TOXICITY
ANIMALS
CHALCOGENIDES
CHEMICAL REACTIONS
COBALT COMPOUNDS
ENERGY
FREE ENERGY
MICROSCOPY
NICKEL COMPOUNDS
PHYSICAL PROPERTIES
SIZE
SULFIDES
SULFUR COMPOUNDS
SURFACE PROPERTIES
THERMODYNAMIC PROPERTIES
TRANSITION ELEMENT COMPOUNDS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology
550300 - Cytology