Structure and expression of the human L-myc gene reveal a complex pattern of alternative mRNA processing
The authors' analyzed in detail the structure of the L-myc gene isolated from human placental DNA and characterized its expression in several small-cell lung cancer cell lines. The gene is composed of three exons and two introns spanning 6.6 kilobases in human DNA. Several distinct mRNA species are produced in all small-cell lung cancer cell lines that express L-myc. These transcripts are generated from a single gene by alternative splicing of introns 1 and 2 and by use of alternative polyadenylation signals. In some mRNAs that is a long open reading frame with a predicted translated protein of 364 residues. Amino acid sequence comparison with c-myc and N-myc demonstrated multiple discrete regions with extensive homology. In contrast, other mRNA transcripts, generated by alternative processing, could encode a truncated protein with a novel carboxy-terminal end.
- Research Organization:
- NCI-Navy Medical Oncology Branch, National Cancer Institute, Naval Hospital, and Uniformed Services Univ. of the Health Sciences, Bethesda, MD (US)
- OSTI ID:
- 6925540
- Journal Information:
- Mol. Cell. Biol.; (United States), Vol. 8:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
NUCLEOPROTEINS
DNA-CLONING
GENETIC MAPPING
RNA PROCESSING
ONCOGENES
MOLECULAR BIOLOGY
AMINO ACID SEQUENCE
CARCINOMAS
CELL CULTURES
CLEAVAGE
DNA
LUNGS
MAN
MESSENGER-RNA
MOLECULAR STRUCTURE
PHENOTYPE
PLACENTA
TUMOR CELLS
ANIMAL CELLS
ANIMALS
BODY
CLONING
CRYSTAL STRUCTURE
DISEASES
FETAL MEMBRANES
GENES
MAMMALS
MAPPING
MEMBRANES
MICROSTRUCTURE
NEOPLASMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PRIMATES
PROTEINS
RESPIRATORY SYSTEM
RNA
VERTEBRATES
550200* - Biochemistry
550400 - Genetics