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Title: Pertussis toxin treatment attenuates some effects of insulin in BC3H-1 murine myocytes

Abstract

The effects of pertussis toxin (PT) treatment on insulin-stimulated myristoyl-diacylglycerol (DAG) generation, hexose transport, and thymidine incorporation were studied in differentiated BC3H-1 mycocytes. Insulin treatment caused a biphasic increase in myristoyl-DAG production which was abolished in myocytes treated with PT. There was no effect of PT treatment on basal (nonstimulated) myristoyl-DAG production. Insulin-stimulated hydrolysis of a membrane phosphatidylinositol glycan was blocked by PT treatment. ADP-ribosylation of BC3H-1 plasma membranes with (/sup 32/P)NAD revealed a 40-kDa protein as the major PT substrate in vivo and in vitro. The time course and dose dependence of the effects of PT on diacylglycerol generation correlated with the in vivo ADP-ribosylation of the 40-kDa substrate. Pertussis toxin treatment resulted in a 71% attenuation of insulin-stimulated hexose uptake without effect on either basal or phorbol ester-stimulated uptake. The stimulatory effects of insulin and fetal calf serum on (/sup 3/H)thymidine incorporation into quiescent myocytes were attenuated by 61 and 59%, respectively, when PT was added coincidently with the growth factors. Nonstimulated and EGF-stimulated (/sup 3/H)thymidine incorporation was unaffected by PT treatment. These data suggest that a PT-sensitive G protein is involved in the cellular signaling mechanisms of insulin.

Authors:
; ; ;
Publication Date:
Research Org.:
Univ. of Virginia School of Medicine, Charlottesville (USA)
OSTI Identifier:
6925014
Resource Type:
Journal Article
Journal Name:
J. Biol. Chem.; (United States)
Additional Journal Information:
Journal Volume: 263:13
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; INSULIN; BIOCHEMISTRY; TOXINS; BIOLOGICAL EFFECTS; TRITIUM COMPOUNDS; UPTAKE; ANIMAL CELLS; CELL DIFFERENTIATION; DOSE-RESPONSE RELATIONSHIPS; GLUCOSE; MICE; MUSCLES; TETRADECANOIC ACID; THYMIDINE; ALDEHYDES; ANIMALS; ANTIGENS; AZINES; CARBOHYDRATES; CARBOXYLIC ACIDS; CHEMISTRY; HETEROCYCLIC COMPOUNDS; HEXOSES; HORMONES; LABELLED COMPOUNDS; MAMMALS; MATERIALS; MONOCARBOXYLIC ACIDS; MONOSACCHARIDES; NUCLEOSIDES; NUCLEOTIDES; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PEPTIDE HORMONES; PYRIMIDINES; RIBOSIDES; RODENTS; SACCHARIDES; TOXIC MATERIALS; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Luttrell, L M, Hewlett, E L, Romero, G, and Rogol, A D. Pertussis toxin treatment attenuates some effects of insulin in BC3H-1 murine myocytes. United States: N. p., 1988. Web.
Luttrell, L M, Hewlett, E L, Romero, G, & Rogol, A D. Pertussis toxin treatment attenuates some effects of insulin in BC3H-1 murine myocytes. United States.
Luttrell, L M, Hewlett, E L, Romero, G, and Rogol, A D. 1988. "Pertussis toxin treatment attenuates some effects of insulin in BC3H-1 murine myocytes". United States.
@article{osti_6925014,
title = {Pertussis toxin treatment attenuates some effects of insulin in BC3H-1 murine myocytes},
author = {Luttrell, L M and Hewlett, E L and Romero, G and Rogol, A D},
abstractNote = {The effects of pertussis toxin (PT) treatment on insulin-stimulated myristoyl-diacylglycerol (DAG) generation, hexose transport, and thymidine incorporation were studied in differentiated BC3H-1 mycocytes. Insulin treatment caused a biphasic increase in myristoyl-DAG production which was abolished in myocytes treated with PT. There was no effect of PT treatment on basal (nonstimulated) myristoyl-DAG production. Insulin-stimulated hydrolysis of a membrane phosphatidylinositol glycan was blocked by PT treatment. ADP-ribosylation of BC3H-1 plasma membranes with (/sup 32/P)NAD revealed a 40-kDa protein as the major PT substrate in vivo and in vitro. The time course and dose dependence of the effects of PT on diacylglycerol generation correlated with the in vivo ADP-ribosylation of the 40-kDa substrate. Pertussis toxin treatment resulted in a 71% attenuation of insulin-stimulated hexose uptake without effect on either basal or phorbol ester-stimulated uptake. The stimulatory effects of insulin and fetal calf serum on (/sup 3/H)thymidine incorporation into quiescent myocytes were attenuated by 61 and 59%, respectively, when PT was added coincidently with the growth factors. Nonstimulated and EGF-stimulated (/sup 3/H)thymidine incorporation was unaffected by PT treatment. These data suggest that a PT-sensitive G protein is involved in the cellular signaling mechanisms of insulin.},
doi = {},
url = {https://www.osti.gov/biblio/6925014}, journal = {J. Biol. Chem.; (United States)},
number = ,
volume = 263:13,
place = {United States},
year = {Thu May 05 00:00:00 EDT 1988},
month = {Thu May 05 00:00:00 EDT 1988}
}