Partial characterization of endogenous digoxinlike substance in human urine
Urinary samples were collected from individuals not taking cardiac glycosides. Aliquots of 30 ml were passed through preparative octadecylsilane-bonded phase columns and eluted in fractions by stepwise increasing concentrations of acetonitrile. Eluted fractions were analysed for their contents of endogenous digoxinlike substance (EDLS) by radioimmunoassay of digoxin and by a bioassay of cardiac glycosides, which measures the uptake of rubidium (/sup 86/Rb) by erythrocytes as an index of Na+, K+-ATPase activity. In both assays, digoxinlike activity was found in several fractions, but the highest values were consistently measured in the fractions eluted with 40% acetonitrile. Greater amounts of EDLS were recovered from the urine of pregnant women than from the urine of men and nonpregnant women.
- Research Organization:
- University Hospital, Lund (Sweden)
- OSTI ID:
- 6924633
- Journal Information:
- Ther. Drug Monit.; (United States), Vol. 10:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DIGOXIN
RADIOIMMUNOASSAY
ATP-ASE
ERYTHROCYTES
MAN
PREGNANCY
RUBIDIUM 86
URINE
ACID ANHYDRASES
ALKALI METAL ISOTOPES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
DAYS LIVING RADIOISOTOPES
DIGITALIS GLYCOSIDES
DRUGS
ENZYMES
GLYCOSIDES
HYDROLASES
IMMUNOASSAY
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MATERIALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHOHYDROLASES
PRIMATES
RADIOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
RUBIDIUM ISOTOPES
TRACER TECHNIQUES
VERTEBRATES
WASTES
550201* - Biochemistry- Tracer Techniques