skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Membrane alterations following toxic chemical insult. Research progress report No. 3 (Final), 15 July 1984-31 January 1988

Abstract

A procaryotic cell system was developed that can be used to determine the toxic action of chemicals acting at the level of the eucaryotic or procaryotic cytoplasmic membrane. Cell wall-less microbes known as mycoplasmas were used. In this current study, two perfluorinated fatty acids (CB and C10) were found to inhibit the growth of the test mycoplasmas. Two apparent activities, cytotoxicity and cytolysis, were observed. At high concentrations (>10 mM), a detergent-like action was noted. At low concentrations (<10 mM), cell death was observed without detectable cell lysis. Altering the cell membrane (the presumed target of the toxic compounds) resulted in altered levels to toxicity. Similar results were obtained when human or murine B-cells were used as the target organism. The toxic action of the perfluorinated fatty acids apparently involves some interaction with the membrane of the cells being treated.

Authors:
Publication Date:
Research Org.:
State Univ. of New York, Binghamton (USA). Dept. of Biological Sciences
OSTI Identifier:
6923713
Report Number(s):
AD-A-194391/9/XAB
Resource Type:
Technical Report
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CARBOXYLIC ACIDS; ORGANIC FLUORINE COMPOUNDS; CYTOLOGY; TOXICITY; TOXIC MATERIALS; BIOLOGICAL EFFECTS; CELL MEMBRANES; CYTOPLASM; DEATH; MYCOPLASMA; PROGRESS REPORT; BIOLOGY; CELL CONSTITUENTS; DOCUMENT TYPES; MATERIALS; MEMBRANES; MICROORGANISMS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC HALOGEN COMPOUNDS; 550900* - Pathology; 550300 - Cytology

Citation Formats

Liss, A. Membrane alterations following toxic chemical insult. Research progress report No. 3 (Final), 15 July 1984-31 January 1988. United States: N. p., 1988. Web.
Liss, A. Membrane alterations following toxic chemical insult. Research progress report No. 3 (Final), 15 July 1984-31 January 1988. United States.
Liss, A. 1988. "Membrane alterations following toxic chemical insult. Research progress report No. 3 (Final), 15 July 1984-31 January 1988". United States.
@article{osti_6923713,
title = {Membrane alterations following toxic chemical insult. Research progress report No. 3 (Final), 15 July 1984-31 January 1988},
author = {Liss, A},
abstractNote = {A procaryotic cell system was developed that can be used to determine the toxic action of chemicals acting at the level of the eucaryotic or procaryotic cytoplasmic membrane. Cell wall-less microbes known as mycoplasmas were used. In this current study, two perfluorinated fatty acids (CB and C10) were found to inhibit the growth of the test mycoplasmas. Two apparent activities, cytotoxicity and cytolysis, were observed. At high concentrations (>10 mM), a detergent-like action was noted. At low concentrations (<10 mM), cell death was observed without detectable cell lysis. Altering the cell membrane (the presumed target of the toxic compounds) resulted in altered levels to toxicity. Similar results were obtained when human or murine B-cells were used as the target organism. The toxic action of the perfluorinated fatty acids apparently involves some interaction with the membrane of the cells being treated.},
doi = {},
url = {https://www.osti.gov/biblio/6923713}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Thu Mar 10 00:00:00 EST 1988},
month = {Thu Mar 10 00:00:00 EST 1988}
}

Technical Report:
Other availability
Please see Document Availability for additional information on obtaining the full-text document. Library patrons may search WorldCat to identify libraries that may hold this item. Keep in mind that many technical reports are not cataloged in WorldCat.

Save / Share: