Comparison of ( sup 3 H)Phencyclidine (( sup 3 H)PCP) and ( sup 3 H) N-(1-(2-thienyl) cyclohexyl)piperidine (( sup 3 H)TCP) binding properties to rat and human brain membranes
The investigation of ({sup 3}H)PCP and ({sup 3}H)TCP binding properties to rat cerebrum and cerebellum resulted in the demonstration of multiple binding sites for the two drugs. In the two tissue preparations PCP had a lower affinity than TCP. In membranes from the cerebrum an equal number of high affinity binding sites were present for ({sup 3}H)PCP and ({sup 3}H)TCP. However, low affinity binding sites were two times more numerous for ({sup 3}H)PCP than for ({sup 3}H)TCP. In the cerebellum, the number of high and low affinity sites labeled by the two radioligands was identical, but the number of high affinity sites was about 7 fold lower than in cerebrum. In human cerebral cortex samples ({sup 3}H)TCP also bound to two different sites. The number of high and low affinity sites were 12 and 3 times, respectively, less abundant than in the rat cerebrum. Low affinity sites were of higher affinity than corresponding sites in the rat brain. In the human cerebellum ({sup 3}H)TCP binding parameters were identical to those measured in the same region in the rat.
- OSTI ID:
- 6921859
- Journal Information:
- Life Sciences; (USA), Vol. 45:26; ISSN 0024-3205
- Country of Publication:
- United States
- Language:
- English
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PIPERIDINES
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CELL MEMBRANES
COMPARATIVE EVALUATIONS
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TRACER TECHNIQUES
TRITIUM COMPOUNDS
AMINES
ANIMALS
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CELL CONSTITUENTS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
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KINETICS
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ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
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550201* - Biochemistry- Tracer Techniques