Sensitive radioimmunoassay and enzyme-linked immunosorbent assay for the simultaneous determination of chloroquine and its metabolites in biological fluids

Journal Article · Mon Jan 01 00:00:00 EST 1990 · Journal of Pharmaceutical Sciences; (USA)

DOI:https://doi.org/10.1002/jps.2600790107· OSTI ID:6921392

Escande, C; Chevalier, P; Verdier, F; Bourdon, R ^[1]

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1. Laboratoire de Biochimie-Toxicologie, Paris (France)

Two new methods for the simultaneous determination of chloroquine and its two main metabolites (monodesethylchloroquine and bisdesethylchloroquine) in biological samples, radioimmunoassay (RIA) and enzyme-linked immunosorbent assay (ELISA), are described. Antiserum is produced in rabbits immunized with N-(2-carboxyethyl)desethylchloroquine:protein conjugate. Besides chloroquine, this antiserum recognizes with good affinity the two main metabolites, monodesethylchloroquine and bisdesethylchloroquine (70 and 40% of crossreaction, respectively). Amodiaquine cross reacts by 4.5%; cross reactions with monodesethylamodiaquine, bisdesethylamodiaquine, and other antimalarial drugs are less than 1%. No extraction step or sample preparation is required for either system. Sensitivity limits are, respectively, 0.70 nM (3 pg of chloroquine sulfate measured in 10 microL of plasma sample) for RIA, and 10 nM (22 pg of chloroquine sulfate measured in 5 microL of plasma sample) for ELISA. The interassay coefficients of variation are, respectively, less than 10 and less than 16% for RIA and ELISA in the range 14-410 nM (6-180 ng/mL). The results of both methods are well correlated (r = 0.97) and correlate with spectrophotometry (r = 0.98) and HPLC results (r = 0.93). Because of their high sensitivity, both methods can be used in the case of chloroquine poisoning and in the control of malaria prophylaxis and treatment.

• OSTI ID: 6921392
• Journal Information: Journal of Pharmaceutical Sciences; (USA), Vol. 79:1; ISSN 0022-3549
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
QUINOLINES
IMMUNOASSAY
BODY FLUIDS
ENZYME IMMUNOASSAY
IODINE ISOTOPES
LIQUID COLUMN CHROMATOGRAPHY
MALARIA
METABOLITES
RABBITS
RADIOIMMUNOASSAY
REAGENTS
SPECTROPHOTOMETRY
SWINE
ANIMALS
AROMATICS
AZAARENES
AZINES
BIOASSAY
BIOLOGICAL MATERIALS
CHROMATOGRAPHY
DIAGNOSTIC TECHNIQUES
DISEASES
DOMESTIC ANIMALS
HETEROCYCLIC COMPOUNDS
IMMUNOLOGY
INFECTIOUS DISEASES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MATERIALS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARASITIC DISEASES
PYRIDINES
RADIOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
SEPARATION PROCESSES
TRACER TECHNIQUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques