Total lymphoid irradiation and discordant cardiac xenografts

Journal Article · Mon Jan 01 00:00:00 EST 1990 · Journal of Heart Transplantation; (USA)

OSTI ID:6919638

Kaplan, E; Dresdale, A R; Diehl, J T; Katzen, N A; Aronovitz, M J; Konstam, M A; Payne, D D; Cleveland, R J ^[1]

---

1. Tufts Univ. School of Medicine, Boston, MA (USA)

Total lymphoid irradiation can prolong concordant cardiac xenografts. The effects of total lymphoid irradiation in a discordant xenograft model (guinea pig to rat) were studied with and without adjuvant pharmacologic immunosuppression. Inbred Lewis rats were randomly allocated to one of four groups. Group 1 (n = 6) served as a control group and rats received no immunosuppression. Group 2 (n = 5) received triple-drug therapy that consisted of intraperitoneal azathioprine (2 mg/kg), cyclosporine (20 mg/kg), and methylprednisolone (1 mg/kg) for 1 week before transplantation. Group 3 animals (n = 5) received 15 Gy of total lymphoid irradiation in 12 divided doses over a 3-week period. Group 4 (n = 6) received both triple-drug therapy and total lymphoid irradiation as described for groups 2 and 3. Complement-dependent cytotoxicity assay was performed to determine if a correlation between complement-dependent cytotoxicity and rejection-free interval existed. Rejection was defined as cessation of graft pulsation and was confirmed by histologic test results. Only groups 1 and 2 showed a difference in survival (group 1, 6.9 +/- 1.0 minutes; group 2, 14.2 +/- 2.7 minutes, p = 0.02). Although total lymphoid irradiation did decrease complement-dependent cytotoxicity, linear regression revealed no correlation between complement-dependent cytotoxicity and graft survival (coefficient of correlation, 0.30). Unlike concordant cardiac xenografts, total lymphoid irradiation with or without triple-drug therapy does not prolong graft survival.

• OSTI ID: 6919638
• Journal Information: Journal of Heart Transplantation; (USA), Vol. 9:1; ISSN 0887-2570
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
HEART
TRANSPLANTS
IMMUNITY
RADIOINDUCTION
LYMPHATIC SYSTEM
BIOLOGICAL RADIATION EFFECTS
GRAFT-HOST REACTION
GUINEA PIGS
IMMUNOSUPPRESSIVE DRUGS
IRRADIATION
RATS
SURVIVAL TIME
ANIMALS
BIOLOGICAL EFFECTS
BODY
CARDIOVASCULAR SYSTEM
DRUGS
MAMMALS
ORGANS
RADIATION EFFECTS
RODENTS
VERTEBRATES
560152* - Radiation Effects on Animals- Animals