Effects of ethanol on an intestinal epithelial cell line
- Laboratoire de Gastroenterologie et de Nutrition, U.E.R. de Medecine, Nice (France)
The effect of exposure of an intestinal epithelial cell line to various concentrations of ethanol (217 mM (1%) to 652 mM (3%)) during 24, 48, and 72 hr was investigated in vitro using a rat intestinal epithelial cell line (IRD 98). Incubation of these cells in the presence of ethanol significantly decreased cell growth. This inhibition was accompanied by a strong increase in cellular protein. Stimulation of specific disaccharidases, gamma-glutamyl transferase, and aminopeptidase activities by ethanol was dose- and time-dependent. Ethanol induces a change in the relative proportions of the different lipid classes synthesized; triglycerides, fatty acids, and cholesterol esters were preferentially synthethysed. Our findings show that cell lines are good models for investigation of the effects of ethanol, and that alcohol considerably modifies the functions of intestinal epithelial cells.
- OSTI ID:
- 6919187
- Journal Information:
- Alcoholism: Clinical and Experimental Research; (USA), Vol. 14:1; ISSN 0145-6008
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ANIMAL CELLS
GROWTH
ETHANOL
BIOLOGICAL EFFECTS
AMINOPEPTIDASES
AMINOTRANSFERASES
DNA
ENZYME ACTIVITY
EPITHELIUM
IN VITRO
INHIBITION
INTESTINES
RATS
TIME DEPENDENCE
ALCOHOLS
ANIMAL TISSUES
ANIMALS
BODY
DIGESTIVE SYSTEM
ENZYMES
GASTROINTESTINAL TRACT
HYDROLASES
HYDROXY COMPOUNDS
MAMMALS
NITROGEN TRANSFERASES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HYDROLASES
RODENTS
TISSUES
TRANSFERASES
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology