Double isotopic method using dansyl chloride for the determination of GABA in rat C6 astrocytoma cell cultures

Journal Article · Wed Jan 01 00:00:00 EST 1986 · J. Neurosci. Res.; (United States)

DOI:https://doi.org/10.1002/jnr.490160311· OSTI ID:6917058

Kohl, R L; Quay, W B; Perez-Polo, J R

Methods are described for the quantitative measurement of GABA in culture. The method can be adapted to any amino acid or dansyl-chloride-reactive species. The sensitivity and selectivity of the procedure result from the double isotopic design in which (/sup 14/C)-labeled internal standard was added to the samples before reaction with (3M)-labeled dansyl chloride. Values obtained by ion-exchange amino acid analysis of cultures agree closely with the values obtained by the double isotopic method. This method is sensitive enough to measure GABA intracellularly and the condition medium.

• OSTI ID: 6917058
• Journal Information: J. Neurosci. Res.; (United States), Vol. 3
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
AMINOBUTYRIC ACID
DUAL-ISOTOPE SUBTRACTION TECHNIQUE
CARBON 14 COMPOUNDS
CELL CULTURES
MEASURING METHODS
RATS
TRITIUM COMPOUNDS
TUMOR CELLS
AMINO ACIDS
ANIMAL CELLS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
CARBOXYLIC ACIDS
DRUGS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
NEUROREGULATORS
ORGANIC ACIDS
ORGANIC COMPOUNDS
RODENTS
TRACER TECHNIQUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques