Thymidine incorporation by the microbial community of standing dead Spartina alterniflora

Journal Article · Sat Nov 01 00:00:00 EST 1986 · Appl. Environ. Microbiol.; (United States)

OSTI ID:6916652

Fallon, R D; Newell, S Y

Thymidine incorporation by the microbial community on standing dead leaves of Spartina alterniflora did not obey many of the assumptions inherent in the use of the technique in planktonic systems. Incorporation rates of (methyl-/sup 3/H)thymidine were nonsaturable over a wide concentration range (10/sup 1/ to 10/sup 5/ nM). Owing to metabolism by both fungi and bacteria, a major fraction of the radiolabel (mean, 48%) appeared in protein. Extraction of the radiolabeled macromolecules were inefficient, averaging 8.8%. Based on an empirically derived conversion factor, 4 x 10/sup 18/ cells mol of thymidine/sup -1/, doubling times ranged from 4 to 69 h for the epiphytic bacterial assemblage.

• Research Organization: Univ. of Georgia Marine Institute, Sapelo Island
• OSTI ID: 6916652
• Journal Information: Appl. Environ. Microbiol.; (United States), Vol. 52:5
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BACTERIA
METABOLISM
FUNGI
THYMIDINE
BIOCHEMICAL REACTION KINETICS
GRASS
PROTEINS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
WETLANDS
AQUATIC ECOSYSTEMS
AZINES
ECOSYSTEMS
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MICROORGANISMS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PLANTS
PYRIMIDINES
REACTION KINETICS
RIBOSIDES
550501* - Metabolism- Tracer Techniques