Use of tritiated thymidine as a marker to compare the effects of matrix proteins on adult human vascular endothelial cell attachment: implications for seeding of vascular prostheses

Journal Article · Sat Nov 01 00:00:00 EST 1986 · Surgery; (United States)

OSTI ID:6916098

Hasson, J E; Wiebe, D H; Sharefkin, J B; D'Amore, P A; Abbott, W M

We have developed a technique to measure attachment of adult human vascular endothelial cells to test surfaces with tritiated thymidine used as a marker. With this technique, we measured attachment of adult human vascular endothelial cells to a series of extracellular matrix proteins, including fibronectin-coated (10 micrograms/cm/sup 2/), laminin-coated (10 micrograms/cm/sup 2/), and collagen-coated (1% gelatin) surfaces because of the role of these proteins in promoting cell attachment and growth. For a typical experiment, in the presence of serum, initial attachment (at 1 hour) was greatest on fibronectin-coated (63%) and gelatin-coated (60%) tissue culture plastic (polystyrene) and was least on laminin-coated (28%) or untreated polystyrene (18%). The data suggest that fibronectin, either alone, or with a more complex combination of extracellular components may need to be present on prosthetic surfaces to produce maximal cell attachment and subsequent growth to confluence in vivo. The described method of measuring attachment is independent of surface properties, ensures complete recovery of cells, and will allow systematic exploration of those properties that best support human endothelial cell attachment to vascular prosthetic surfaces.

• Research Organization: Massachusetts General Hospital, Boston
• OSTI ID: 6916098
• Journal Information: Surgery; (United States), Vol. 5
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
ENDOTHELIUM
ADHESION
PROSTHESES
COLLAGEN
PROTEINS
SURFACES
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VEINS
ANIMAL TISSUES
AZINES
BLOOD VESSELS
BODY
CARDIOVASCULAR SYSTEM
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MEDICAL SUPPLIES
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRIMIDINES
RIBOSIDES
SCLEROPROTEINS
TISSUES
550601* - Medicine- Unsealed Radionuclides in Diagnostics